Adeno-Associated Virus (AAV-DJ)-Cryo-EM Structure at 1.56 Å Resolution.
Adeno-Associated Virus (AAV-DJ)-Cryo-EM Structure at 1.56 Å Resolution.
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分辨率为1.56Å的腺相关病毒（AAV-DJ）-Cryo-EM结构。
DOI：
10.3390/v12101194
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发表时间：
2020-10-20
期刊：
影响因子：
--
通讯作者：
Chapman MS
中科院分区：
文献类型：
--
作者：
Xie Q;Yoshioka CK;Chapman MS
腺相关病毒是基因治疗的主要病毒载体。AAV-DJ是开发用于向肝的重组变体。AAV-DJ结构已通过冷冻电子显微镜（cryo-EM）确定为1.56 μ m分辨率。只有脱铁铁蛋白在1.6 μ m或更高分辨率的预印本中被报道，而AAV-DJ几乎与通过病毒晶体的X射线衍射获得的最高分辨率相匹配。然而，cryo-EM的优点是大多数氢是清晰的，提高了原子细化的准确性，并消除了氢键识别中的模糊性。在氢键是可预测的二级结构之外，来自氢原子和受体原子的直接观察的氢键网络与即使在2.8 μ m分辨率下推断的氢键网络也大不相同。理解病毒组装的意义意味着冷冻电镜可能成为高分辨率结构病毒学的首选方法。
Adeno-associated virus is the leading viral vector for gene therapy. AAV-DJ is a recombinant variant developed for tropism to the liver. The AAV-DJ structure has been determined to 1.56 Å resolution through cryo-electron microscopy (cryo-EM). Only apoferritin is reported in preprints at 1.6 Å or higher resolution, and AAV-DJ nearly matches the highest resolutions ever attained through X-ray diffraction of virus crystals. However, cryo-EM has the advantage that most of the hydrogens are clear, improving the accuracy of atomic refinement, and removing ambiguity in hydrogen bond identification. Outside of secondary structures where hydrogen bonding was predictable a priori, the networks of hydrogen bonds coming from direct observation of hydrogens and acceptor atoms are quite different from those inferred even at 2.8 Å resolution. The implications for understanding viral assembly mean that cryo-EM will likely become the favored approach for high resolution structural virology.
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影响因子：
48
作者：
Kucukelbir, Alp;Sigworth, Fred J.;Tagare, Hemant D.
通讯作者：
Tagare, Hemant D.
影响因子：
64.8
作者：
DEROSIER, DJ;KLUG, A
通讯作者：
KLUG, A
影响因子：
5.6
作者：
Lane, Stephen W.;Dennis, Caitriona A.;Phillips, Simon E. V.
通讯作者：
Phillips, Simon E. V.
DOI：
10.1107/s2059798318006551
发表时间：
2018-06-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Afonine PV;Poon BK;Read RJ;Sobolev OV;Terwilliger TC;Urzhumtsev A;Adams PD
通讯作者：
Adams PD
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL