Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype
Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype
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DOI：
10.1016/j.actbio.2008.04.013
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发表时间：
2008-09-01
影响因子：
9.7
通讯作者：
Hahn, Marlah S.
中科院分区：
文献类型：
--
作者：
Liao, Huimin;Munoz-Pinto, Dany;Hahn, Marlah S.
目前声带（VF）瘢痕的临床管理产生不一致的，往往是次优的结果。研究人员正在研究VF固有层（LP）瘢痕的一些替代治疗方法，包括用于功能性LP再生的设计植入材料。在本研究中，我们研究了初始支架弹性模量和网孔尺寸对包囊VF成纤维细胞（VFF）细胞外基质（ECM）生产的影响，以实现合理的支架设计。聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶被选为这项研究，因为他们的材料性能，包括机械性能，网孔大小，降解速率和生物活性。可以严格控制和系统地修改。将猪VFF封装在四种PEGDA水凝胶中，降解半衰期类似于25天，但初始弹性压缩模量和筛孔尺寸范围分别为类似于30至100 kPa和类似于9至27 rim。在30天的静态培养后，在生物化学和组织学上评估每种制剂中的VFF ECM产生和表型。硫酸化糖胺聚糖的合成增加在相似的程度上与增加初始模量和减少初始网格尺寸。与此相反，弹性蛋白的生产随着初始模量的增加而减少，但随着初始网目尺寸的减小而增加。胶原沉积和诱导成肌纤维细胞表型强烈依赖于初始补片尺寸，但似乎基本上不受初始模量变化的影响。目前的结果表明，支架网孔大小值得进一步研究，作为VFF ECM合成的关键调节剂。此外，本研究验证了一种系统且受控的方法来分析VFF对支架特性的反应，这应该有助于合理的支架选择/设计。(C)2008 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Current clinical management of vocal fold (VF) scarring produces inconsistent and often suboptimal results. Researchers are investigating a number of alternative treatments for VF lamina propria (LP) scarring, including designer implant materials for functional LP regeneration. In the present study, we investigate the effects of the initial scaffold elastic modulus and mesh size on encapsulated VF fibroblast (VFF) extracellular matrix (ECM) production toward rational scaffold design. Poly(ethylene glycol) diacrylate (PEGDA) hydrogels were selected for this study since their material properties, including mechanical properties, mesh size, degradation rate and bioactivity. can be tightly controlled and systematically modified. Porcine VFF were encapsulated in four PEGDA hydrogels with degradation half lives of similar to 25 days, but with initial elastic compressive moduli and mesh sizes ranging from similar to 30 to 100 kPa and from similar to 9 to 27 rim, respectively. After 30 days of static culture, VFF ECM production and phenotype in each formulation was assessed biochemically and histologically. Sulfated glycosaminoglycan synthesis increased in similar degree with both increasing initial modulus and decreasing initial mesh size. In contrast, elastin production decreased with increasing initial modulus but increased with decreasing initial mesh size. Both collagen deposition and the induction of a myofibroblastic phenotype depended strongly on initial mesh size but appeared largely unaffected by variations in initial modulus. The present results indicate that scaffold mesh size warrants further investigation, nation as a critical regulator of VFF ECM synthesis. Furthermore, this study validates a systematic and controlled approach for analyzing VFF response to scaffold properties, which should aid in rational scaffold selection/design. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.