A study of analgesic effect of Zanthoxylum bungeanum Maxim pharmacopuncture
A study of analgesic effect of Zanthoxylum bungeanum Maxim pharmacopuncture
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DOI：
10.13045/acupunct.2017083
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发表时间：
2017-05
期刊：
影响因子：
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通讯作者：
Jung Hee Lee;Y. Lee;Hyun Jong Lee;B. Lee;Jae Soo Kim
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文献类型：
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作者：
Jung Hee Lee;Y. Lee;Hyun Jong Lee;B. Lee;Jae Soo Kim
目的：观察花椒药刺对福尔马林致痛大鼠和ICR小鼠的镇痛作用。研究方法：受试者将8周龄恒定痛敏大鼠分为5组;正常（在Taegye（KI 3）用生理盐水处理，在后爪注射生理盐水之前），Con-1（在后爪注射福尔马林之前，在KI 3下用生理盐水处理），Lido-1（在KI 3时用利多卡因治疗），ZMWG-1（在KI 3处用花椒的热水提取物药物穿刺治疗），ZMEG-1（用花椒乙醇提取物在KI 3处进行药物穿刺）。35分钟后，测定大鼠超声发声（USV）和血清中天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）活性。此外，通过注射5周龄的ICR小鼠进行甩尾试验。按药物种类（生理盐水、利多卡因、ZMW、ZME）分为Con-2、Lido-2、ZMWG-2、ZMEG-2 4组。在每次药物注射后，我们通过将尾部置于50°C的水中来检查反应。结果：ZME对甲醛致大鼠足背静脉痛的早、晚期均有镇痛作用。ZMEG-1和Lido-1在USV的早期和晚期无显著差异。此外，与Con-1相比，ZMWG-1和ZMEG-1中的血清AST和ALT活性未观察到显著差异。对于甩尾试验，与Con-2相比，Lido-2和ZMEG-2对温暖的镇痛作用显著增加。结论：ZME药刺对福尔马林致痛有镇痛作用，且无肝毒性。此外，甩尾实验表明，ZME药物针刺可以用于镇痛作用的温暖和治疗疼痛的技术。
Objectives :This study was carried out to evaluate analgesic effects of Zanthoxylum bungeanum Maxim (ZM) pharmacopuncture on formalin-induced pains in Sprague-Dawley (SD) rats and ICR-mice. Methods : The subjects were divided 8 weeks aged rats with constant pain sensitivity into five groups; normal (treated with normal saline at Taegye (KI3) and before injected with normal saline at hindpaw), Con-1 (treated with normal saline at KI3 before injected with formalin at hindpaw), Lido-1 (treated with lidocaine at KI3), ZMWG-1 (treated with Hot water extraction pharmacopuncture of Zanthoxylum bungeanum Maxim at KI3), ZMEG-1 (treated with ethanol extraction pharmacopuncture of Zanthoxylum bungeanum Maxim at KI3). After 35 minutes, we measured ultrasonic vocalization (USV) and enzyme activities of both Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) in rat serum. In addition, Tail flick test is performed by injecting ICR mice at 5 weeks of age. And it classified into 4 groups (Con-2, Lido-2, ZMWG-2, ZMEG-2) according to the kind of drug (normal saline, lidocaine, ZMW, ZME). After each drug injection, we examined the reaction by placing the tail in water at 50°C. Results : ZME had analgesic effects in the early and late phase of USV during the formalin test. There were no significant differences between ZMEG-1 and Lido-1 in early and late phase of USV. Also, No significant differences observed in serum AST and ALT activity in ZMWG-1 and ZMEG-1 compared with Con-1. For tail-flick test, analgesic effect on warmth significantly increased in Lido-2 and ZMEG-2 compare to that of Con-2. Conclusion : ZME pharmacopuncture had analgesic effects on formalin-induced pain without liver toxicity. Also, tail-flick test suggest that ZME pharmacopuncture could be useful technique on analgesic effect on warmth and treatment of pains.