High-fluorescent carbon dots (CDs) originated from China grass carp scales (CGCS) for effective detection of Hg(II) ions
High-fluorescent carbon dots (CDs) originated from China grass carp scales (CGCS) for effective detection of Hg(II) ions
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DOI：
10.1016/j.microc.2018.11.044
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发表时间：
2019-03-01
影响因子：
4.8
通讯作者：
Song, Youtao
中科院分区：
文献类型：
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作者：
Liu, Guanhong;Jia, Haishuang;Song, Youtao
碳点作为一种环境友好型荧光纳米材料，在生物成像、生物传感器等领域有着广泛而潜在的应用前景，受到了人们的广泛关注。值得一提的是，从含碳废物的回收中制备镉已经引起了相当大的研究兴趣。提出了一种简单的碳点合成技术，即以中国草鱼鳞片为原料，采用微波水热法一步法制备了硫化镉。由于汞离子具有较强的亲硫性，S原子表现出较高的热力学亲和力和较快的与Hg2+离子的结合过程。由于CGCS-CDS中含有大量的含半胱氨酸的巯基，可以作为检测Hg2+离子的特异性荧光探针。随后，用高分辨透射电子显微镜、X射线衍射仪、傅立叶变换红外光谱和X射线光电子能谱等手段对CGCS-CDS进行了表征。通过荧光光谱和紫外-可见光谱表征了CGCS-CDS的光学性质。此外，还通过荧光光谱研究了pH值和盐浓度对CGCS-CDS光学性质的影响。在最佳条件下，荧光强度的降低与汞离子的允许检测浓度在0.014-30 mU/L范围内呈良好的线性关系，检出限为0.014 mU/L。同时，研究了其他金属离子对CGCS-CDS作为荧光探针检测Hg2+离子的影响，表明CGCS-CDS是一种良好的检测Hg2+离子的荧光探针。最终，CGCS-CDS被证明是低毒的，并被应用于湖水和化妆品中Hg2+的检测，展示了它们的潜在应用前景。
Carbon dots (CDs) as a kind of environment-friendly fluorescent nanomaterial has been actively studied because of wide and potential applications, such as bioimaging and biosensors. It is worth mentioning that preparing CDs from the recycling of carbonaceous waste has received considerable research interest. We proposed a simple carbon dots synthesis technique, that is, using China grass carp scales (CGCS) as original and green materials by a one-step microwave hydrothermal method to prepare CDs. Since the mercury ion has strongly sulphophile, S atoms display higher thermodynamic affinity and faster integrating process with Hg2+ ions. Due to the presence of large amount of cysteine-containing sulfhydryl groups, the obtained CGCS-CDs can be used as a specific fluorescence probe for detecting Hg2+ ions. Subsequently, CGCS-CDs were characterized by HR-TEM, XRD, FT-IR and XPS analyses. The optical properties of CGCS-CDs were elucidated by fluorescence and UV-vis spectra. Additionally, the effects of pH values and salt concentrations on optical properties of CGCS-CDs were also researched by fluorescence spectra. Under optimal conditions, the decrease of fluorescence intensity displays a good linear relationship with allowable Hg2+ ion detection concentration range of 0.014-30 mu mol/L and a limit of detection (LOD) of 0.014 mu mol/L is acquired. Meanwhile, the effect of other metal ions on the detection of Hg2+ ions by using CGCS-CDs as fluorescence probe was studied, suggesting that the CGCS-CDs could be an excellent fluorescence probe for the detection of Hg2+ ions. Eventually, the CGCS-CDs were proven to be low toxicity and applied for Hg2+ ions detection in lake water and cosmetic, demonstrating their potential towards diverse applications.