Cellular and humoral responses to influenza in Gabonese children living in rural and semi-urban areas
Cellular and humoral responses to influenza in Gabonese children living in rural and semi-urban areas
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DOI：
10.1086/522010
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发表时间：
2007-12-01
影响因子：
6.4
通讯作者：
Yazdanbakhsh, M.
中科院分区：
文献类型：
--
作者：
Van Riet, E.;Adegnika, A. A.;Yazdanbakhsh, M.
背景随着目前对禽流感病毒大流行威胁的关注，人们认识到，关于非洲流感的信息很少。此外，在非洲国家接种流感疫苗的效果可能与接触微生物和寄生虫较少的地区的效果非常不同。为了监测流感病毒的存在并调查对流感疫苗接种的免疫反应，对加蓬半城市和农村地区的学龄儿童进行了研究。在血清中测定对疫苗中代表的3种菌株的流感特异性抗体应答。此外，在接种疫苗前后，在流感抗原体外刺激全血后，测量细胞因子应答。接种前H3 N2抗体滴度高。在接种疫苗时，针对3种流感病毒株的抗体滴度显著增加。接种疫苗后，农村学生的抗H1N1和抗B反应弱于半城市学生。流感特异性细胞因子反应在一周内被诱导，显示来自农村地区的儿童中干扰素-γ显著较低，白细胞介素-5显著较高。疫苗接种前的抗体水平表明，流感病毒在加蓬流行。总之，流感疫苗接种诱导较弱的免疫反应，在农村人口比在半城市人口的加蓬学童。
Background. With the current attention to the pandemic threat of avian influenza viruses, it is recognized that there is little information on influenza in Africa. In addition, the effects of influenza vaccination in African countries could be very different from the effects in regions with less exposure to microorganisms and parasites.Methods. To monitor the presence of influenza viruses and investigate the immunological responses to influenza vaccination, schoolchildren in semi-urban and rural regions of Gabon were studied. Influenza-specific antibody responses to the 3 strains represented in the vaccine were determined in the serum. Furthermore, cytokine responses were measured after in vitro stimulation of whole blood by influenza antigens, before and after vaccination.Results. Prevaccination titers of antibody against H3N2 were high. At vaccination, the titers of antibody against the 3 influenza strains increased significantly. The anti-H1N1 and anti-B responses after vaccination were weaker in rural schoolchildren than in semi-urban schoolchildren. Influenza-specific cytokine responses were induced within a week, showing significantly lower interferon-gamma and significantly higher interleukin-5 in the children from rural areas.Conclusions. Prevaccination antibody levels indicated that influenza viruses circulate in Gabon. Altogether, influenza vaccination induces weaker immune responses in a rural population than in a semi-urban population of Gabonese schoolchildren.