Characterization of antibodies specific for hemagglutinin and neuraminidase proteins of the 1918 and 2009 pandemic H1N1 viruses
Characterization of antibodies specific for hemagglutinin and neuraminidase proteins of the 1918 and 2009 pandemic H1N1 viruses
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1918 年和 2009 年大流行 H1N1 病毒的血凝素和神经氨酸酶蛋白特异性抗体的表征。
DOI：
10.1016/j.vaccine.2010.10.059
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发表时间：
2010-12-16
期刊：
影响因子：
5.5
通讯作者：
Qi, Zhongtian
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Liu, Xiaoqing;Qi, Zhongtian
血清学研究发现，一些人对2009年大流行性H1N1流感病毒（H1N1-2009）具有保护性免疫。然而，由于人类免疫背景的复杂性，对预先存在的免疫的进一步研究变得复杂。在这里，我们用HA-和na编码质粒免疫小鼠。采用H1N1-1918和2009假型粒子（pps）和H1N1-2009分离株，评价了抗ha抗血清的交叉中和活性和抗na抗血清对病毒感染的影响。H1N1-2009 HA （09HA）抗体可中和含有09HA或H1N1-1918 HA （18HA）的pps；同样，18HA抗体可中和含有18HA或09HA的pps。09HA和18HA抗体也能高效中和H1N1-2009病毒。甲型H1N1-1918 NA （18NA）抗体和甲型H1N1-2009 NA （09NA）抗体均能增强携带09NA和18NA的pps的传染性。尽管抗09na和-18NA抗体在多周期感染试验中显著降低了细胞病变效应，相反，这些抗体在单周期感染试验中增强了H1N1-2009的传染性。我们的研究表明，针对这两种抗原性相关病毒株的抗体之间存在交叉保护，并表明抗na抗体具有双重作用，需要重新检查其在人类免疫中的作用。(C) 2010 Elsevier Ltd.版权所有。
Serologic studies have detected protective immunity against 2009 pandemic H1N1 influenza virus (H1N1-2009) in some people. However, further study of preexisting immunity has been complicated by the complexity of the human immunological background. Here, we immunized mice with HA- and NA-encoding plasmids. The cross-neutralizing activity of the anti-HA antisera and the effect of the anti-NA antisera on viral infectivity were evaluated using H1N1-1918- and 2009-pseudotyped particles (pps) and an H1N1-2009 isolate. Antibodies to H1N1-2009 HA (09HA) neutralized pps harboring 09HA or H1N1-1918 HA (18HA): similarly, antibodies to 18HA neutralized pps harboring 18HA or 09HA. Antibodies to 09HA and 18HA also neutralized the H1N1-2009 virus with high efficiency. Antibodies to H1N1-1918 NA (18NA) or H1N1-2009 NA (09NA) both enhanced the infectivity of pps harboring 09NA and 18NA. Although anti-09NA and -18NA antibodies significantly reduced cytopathic effects in multiple-cycle infection assays, conversely, these antibodies enhanced the infectivity of H1N1-2009 in single-cycle infection assays. Our study demonstrates the existence of cross-protection between antibodies against these two antigenically related virus strains and shows that anti-NA antibodies have a dual effect that requires reexamination of their role in human immunity. (C) 2010 Elsevier Ltd. All rights reserved.