Ellagic acid improves endogenous neural stem cells proliferation and neurorestoration through Wnt/β-catenin signaling in vivo and in vitro
Ellagic acid improves endogenous neural stem cells proliferation and neurorestoration through Wnt/β-catenin signaling in vivo and in vitro
复制标题
鞣花酸通过体内和体外 Wnt/β-catenin 信号传导改善内源性神经干细胞增殖和神经恢复
DOI：
10.1002/mnfr.201600587
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发表时间：
2017-03-01
影响因子：
5.2
通讯作者：
Pang, Zongran
中科院分区：
文献类型：
--
作者：
Liu, Qing-Shan;Li, Shu-Ran;Pang, Zongran
适用范围：本研究的目的是研究多酚鞣花酸（EA）对脑细胞的影响，并探讨其作用机制，并评估EA是否可以安全地利用人类作为一种功能性食品或治疗agent.Methods和结果：光血栓形成诱导的大鼠脑损伤模型的创建，和EA灌胃给大鼠静脉缺血后连续7天。建立神经干细胞缺氧缺糖再灌注模型，观察电针对神经干细胞增殖的影响。电针可改善脑缺血大鼠的神经相关功能，改善脑梗死体积和形态学改变，提高脑半暗区nestin蛋白含量。结论：电针可通过Wnt/beta-catenin信号通路促进神经干细胞的增殖，改善脑损伤后神经干细胞的预后。所提出的结果代表了新的见解EA的脑细胞保护活性的机制。因此，EA可用于功能性食品或药物中，以帮助治疗神经功能障碍，神经退行性疾病和衰老。
Scope: The aim of this study is to research the effects of the polyphenol ellagic acid (EA) on brain cells and to explore its mechanism of action, and to evaluate whether EA can be safely utilized by humans as a functional food or therapeutic agent.Methods and results: A photothrombosis-induced model of brain injury in rats was created, and EA was administered intragastrically to rats on 7 consecutive days post-venous ischemia. An oxygen-glucose deprivation and re-perfusion model was established in neural stem cells in order to research the effects on proliferation after 2 days of EA treatment in vitro. The administration of EA improved the rats' nerve-related abilities, remedied infarct volumes and morphological changes in the brain, and enhanced the content of nestin protein in the brain semidarkness zone. The proliferation of NSCs and the expression of beta-catenin and Cyclin D1 genes were also increased in primary cultured NSCs.Conclusions: EA administration can improve brain injury outcomes and increase the proliferation of NSCs through the Wnt/beta-catenin signaling pathway. The presented results represent new insights on the mechanisms of the brain cell protective activity of EA. Thus, EA may be used in functional foods or medicines to help treat nerve dysfunction, neurodegenerative disease and aging.