Antimicrobial activity of synthetic salivary peptides against voice prosthetic microorganisms
Antimicrobial activity of synthetic salivary peptides against voice prosthetic microorganisms
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DOI：
10.1097/00005537-200002010-00027
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发表时间：
2000-02-01
期刊：
影响因子：
2.6
通讯作者：
Albers, FWJ
中科院分区：
文献类型：
--
作者：
Elving, GJ;van der Mei, HC;Albers, FWJ
目的：研究合成唾液抗菌肽是否对从使用过的硅橡胶发声器中分离出的细菌和酵母菌的生长具有抑制作用。方法：测定浓度为 2 和 4 mg/mL 的六种合成唾液肽（组氨酸 5、dhvar1、dhvar4、dhvar5、乳铁蛋白 b 17-30 [LFb 17-30] 和半胱氨酸蛋白酶抑制剂 51-15）针对不同口咽酵母菌（四种）和细菌（八种）菌株以及针对“总菌株”的抗菌活性。 使用琼脂扩散测试从移植的发声假体中分离出微生物群”。定性确定敏感微生物的谱。结果：组蛋白 5 和胱抑素 51-15 对本研究中涉及的微生物没有表现出任何抗菌活性。 Dhvar1 对一些测试的口咽部微生物（包括酵母菌株）具有活性，但对 Rothia dentocariosa、金黄色葡萄球菌、大肠杆菌和总微生物群落没有活性。 Dhvar4 对所有测试的微生物（包括总微生物群）都有活性，而 Dhvar5 对大肠杆菌和总微生物群缺乏活性。 LFb 17-30 不会抑制任何相关酵母菌株的生长，并且仅对某些细菌菌株表现出较小的活性。 LFb 17-30 轻微抑制外植假体总微生物群的生长。结论：合成唾液肽 dhvar1 对通常从外植发声假体中分离出的所有微生物（包括酵母菌）具有广泛的抗菌活性。因此，它可能是一种有用的药物，作为抗生素和抗真菌药的替代品，以各种方式使用，以延长喉切除术患者的发声假体的使用寿命。
Objectives: To investigate whether synthetic salivary antimicrobial peptides have an inhibitory effect on the growth of bacteria and yeasts isolated from used silicone rubber voice prostheses. Methods: The antimicrobial activities of six synthetic salivary peptides (histatin 5, dhvar1, dhvar4, dhvar5, lactoferrin b 17-30 [LFb 17-30], and cystatin 51-15) at concentrations of 2 and 4 mg/mL were determined against different oropharyngeal yeast (four) and bacterial (eight) strains and against a "total microflora" isolated from explanted voice prostheses using agar diffusion tests. The spectrum of susceptible microorganisms was determined qualitatively. Results: Histatin 5 and cystatin 51-15 did not show any antimicrobial activity against the microorganisms involved in this study. Dhvar1 was active against some of the oropharyngeal microorganisms tested, including the yeast strains, but not against Rothia dentocariosa, Staphylococcus aureus, Escherichia coli and the total microflora. Dhvar4 was active against all microorganisms tested, including the total microflora, Dhvar5 lacked activity against E coli and the total microflora. LFb 17-30 did not inhibit the growth of any of the yeast strains involved and showed only minor activity against some of the bacterial strains. LFb 17-30 slightly inhibited the growth of the total microflora from an explanted prosthesis. Conclusions: The synthetic salivary peptide dhvar1 has a broad antimicrobial activity against all microorganism that are commonly isolated from explanted voice prostheses, including yeasts. Therewith, it may represent a useful drug, as an alternative for antibiotics and antimycotics employed in various ways to prolong the Lifetime of voice prostheses in laryngectomees.