Nitrogen dioxide radical generated by the myeloperoxidase-hydrogen peroxide-nitrite system promotes lipid peroxidation of low density lipoprotein
Nitrogen dioxide radical generated by the myeloperoxidase-hydrogen peroxide-nitrite system promotes lipid peroxidation of low density lipoprotein
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DOI：
10.1016/s0014-5793(99)00893-5
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发表时间：
1999-07-23
期刊：
影响因子：
3.5
通讯作者：
Heinecke, JW
中科院分区：
文献类型：
--
作者：
Byun, J;Mueller, DM;Heinecke, JW
髓过氧化物酶是一种由活化的吞噬细胞分泌的血红素蛋白，在人类动脉粥样硬化病变中存在并具有酶活性。在目前的研究中，我探索了髓过氧化物酶产生的活性氮物质促进低密度脂蛋白（LDL）脂质过氧化的可能性-这种修饰可能使脂蛋白致动脉粥样硬化。我们发现髓过氧化物酶，H2 O2生成系统和亚硝酸盐（NO2-）过氧化LDL脂质。该过程需要NO2-和酶系统的每个组件;它，vas抑制过氧化氢酶，氰化物和抗坏血酸，水相自由基的有效清除剂。LDL的过氧化作用不需要氯离子的参与，而次氯酸清除剂牛磺酸对LDL的过氧化作用的影响很小。总之，这些结果表明，脂质过氧化作用是由一氧化氮自由基类物质促进的。这些观察结果表明，髓过氧化物酶，凭借其形成活性氮中间体的能力，可能会促进脂质过氧化和动脉粥样硬化，（C）1999年欧洲生物化学学会联合会。
Myeloperoxidase, a heme protein secreted by activated phagocytes, is present and enzymatically active in human atherosclerotic lesions, In the current studies, me explored the possibility that reactive nitrogen species generated by myeloperoxidase promote lipid peroxidation of low density lipoprotein (LDL) - a modification that may render the lipoprotein atherogenic, We found that myeloperoxidase, an H2O2-generating system and nitrite (NO2-) peroxidized LDL lipids. The process required NO2- and each component of the enzymatic system; it,vas inhibited by catalase, cyanide and ascorbate, a potent scavenger of aqueous phase radicals. LDL, peroxidation did not require chloride ion, and it was little affected by the hypochlorous acid scavenger taurine, Collectively, these results suggest that lipid peroxidation is promoted by a nitrogen dioxide radical-like species. These observations indicate that myeloperoxidase, by virtue of its ability to form reactive nitrogen intermediates, may promote lipid peroxidation and atherogenesis, (C) 1999 Federation of European Biochemical Societies.