Antibiofilm Activities of a Novel Chimeolysin against Streptococcus mutans under Physiological and Cariogenic Conditions.
Antibiofilm Activities of a Novel Chimeolysin against Streptococcus mutans under Physiological and Cariogenic Conditions.
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新型 Chimeolysin 在生理和致龋条件下对变形链球菌的抗菌膜活性。
DOI：
10.1128/aac.01872-16
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发表时间：
2016
影响因子：
4.9
通讯作者：
Wei Hongping
中科院分区：
文献类型：
--
作者：
Yang Hang;Bi Yongli;Shang Xiaoran;Wang Mengyue;Linden Sara B;Li Yunpeng;Li Yuhong;Nelson Daniel C;Wei Hongping
变形链球菌通常以生物膜形式存在于牙齿表面，并促进龋齿的发展。我们研究了ClyR，一种工程化的溶血素，对S。在生理和致龋条件下的变形菌生物膜。药敏试验表明，ClyR对所有临床沙门氏菌均有抗菌活性。变异株以及S.变形杆菌生物膜显示出对青霉素的抗性。色葡萄用100 μg/ml ClyR处理后，在生理糖条件和致龋条件下在羟基磷灰石盘上形成的变形杆菌生物膜分别减少了约21 og和31 og。相比之下，在葡萄糖酸氯己定（ChX）处理组中仅观察到1个对数减少，并且在NaF处理组中未观察到杀伤作用。小鼠牙齿定植模型显示，重复使用ClyR 3周（5 μg/天）可减少定植的S.对小鼠牙菌斑中的变形细胞无明显影响（P> 0.05）。此外，在超过体外和体内研究所用浓度时未观察到毒性，在口腔中重复使用ClyR后，在小鼠唾液中无法检测到ClyR特异性抗体。我们的数据共同证明ClyR对S有活性。在体外和体内均存在变异体生物膜，因此代表了用于对抗龋齿的预防剂或治疗剂。
Streptococcus mutans often survives as a biofilm on the tooth surface and contributes to the development of dental caries. We investigated the efficacy of ClyR, an engineered chimeolysin, against S. mutans biofilms under physiological and cariogenic conditions. Susceptibility tests showed that ClyR was active against all clinical S. mutans isolates tested as well as S. mutans biofilms that displayed resistance to penicillin. The S. mutans biofilms that formed on hydroxyapatite discs under physiological sugar conditions and cariogenic conditions were reduced ∼2 logs and 3 logs after treatment with 100 μg/ml ClyR, respectively. In comparison, only a 1-log reduction was observed in the chlorhexidine gluconate (ChX)-treated group, and no killing effect was observed in the NaF-treated group. A mouse dental colonization model showed that repeated use of ClyR for 3 weeks (5 μg/day) reduced the number of colonized S. mutans cells in the dental plaques significantly (P< 0.05) and had no harmful effects on the mice. Furthermore, toxicity was not noted at concentrations exceeding those used for thein vitroandin vivostudies, and ClyR-specific antibodies could not be detected in mouse saliva after repeated use of ClyR in the oral cavity. Our data collectively demonstrate that ClyR is active against S. mutans biofilms bothin vitroandin vivo, thus representing a preventative or therapeutic agent for use against dental caries.