An organic indicator functionalized graphene oxide nanocomposite-based colorimetric assay for the detection of sarcosine
An organic indicator functionalized graphene oxide nanocomposite-based colorimetric assay for the detection of sarcosine
复制标题
用于检测肌氨酸的有机指示剂功能化氧化石墨烯纳米复合材料比色法
DOI：
10.1039/c6nr00005c
复制
发表时间：
2016
期刊：
影响因子：
6.7
通讯作者：
Lu Xiaoquan
中科院分区：
文献类型：
--
作者：
Xue Zhonghua;Yin Bo;Wang Hui;Li Mengqian;Rao Honghong;Liu Xiuhui;Zhoua Xinbin;Lu Xiaoquan
肌氨酸的快速检测是疾病诊断和治疗的关键要求。我们在这里报告了一个简单而灵敏的比色纳米复合平台，用于快速检测碱性介质中的肌氨酸。该方法利用了有机指示剂1，2-萘醌-4-磺酸钠盐（NQS）和肌氨酸物质的分析物之间的快速显色反应的优点，以及氧化石墨烯（GO）由于其良好的水溶性、极大的表面积和容易的表面改性而形成高度着色的产物的良好催化能力。因此，通过π-π堆叠的NQS官能化GO纳米复合材料已被证明可用作高效催化剂体系，用于通过提供纳米复合材料放大的比色响应来选择性和灵敏地比色测定肌氨酸。同时，该方法对肌氨酸的选择性好，检测限为0.73 μM。更重要的是，通过使用电化学方法，可以容易地验证和阐明用于特定分析物测定的基于GO纳米复合物的比色平台的可靠传感机制，这也提供了传统表征策略的有吸引力的替代方案。
Rapid detection of sarcosine is a key requirement for both diagnosis and treatment of disease. We report here a simple yet sensitive colorimetric nanocomposite platform for rapid detection of sarcosine in alkaline media. The approach exploited the benefits of a rapid color-producing reaction between an organic indicator, 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS), and the analyte of sarcosine species as well as the good catalytic ability of graphene oxide (GO) to the formation of highly colored products due to its good water dispersibility, extremely large surface area and facile surface modification. As a result, a NQS functionalized GO nanocomposite through π–π stacking has been demonstrated to be useful as a highly efficient catalyst system for the selective and sensitive colorimetric determination of sarcosine by providing a nanocomposite-amplified colorimetric response. Meanwhile, the strategy offered excellent selectivity toward sarcosine species against other amino acids as well as a satisfying detection limit of 0.73 μM. More importantly, by using an electrochemical method, a credible sensing mechanism of GO nanocomposite-based colorimetric platform for a special analyte determination can be easily verified and elucidated, which also provides an attractive alternative to conventional characterization strategies.