Decoding active components in a formulation of multiple herbs for treatment of psoriasis based on three cell lines fishing and liquid chromatography-mass spectrometry analysis
Decoding active components in a formulation of multiple herbs for treatment of psoriasis based on three cell lines fishing and liquid chromatography-mass spectrometry analysis
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DOI：
10.1016/j.jpba.2020.113331
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发表时间：
2020-07-15
影响因子：
3.4
通讯作者：
Li, Shaoping
中科院分区：
文献类型：
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作者：
Chen, Lingxiao;Chen, Haiming;Li, Shaoping
中药方剂通常含有多种药物，成分复杂，其疗效是多种成分综合作用的结果。因此，对配方中的生物活性化合物进行筛选是必要的，尽管这非常困难，不仅对于阐明治疗成分，而且对于制定质量控制方法也是必要的。本研究采用三种细胞系钓鱼法结合LC-MS分析筛选临床治疗银屑病的中药复方扶正止痒和复止痒汤中潜在的活性成分。共发现19个潜在活性成分，其中13个分别鉴定为野蔷薇苷D、蜂毒苷或野蔷薇苷A、tufulingoside、新落新妇苷、落新妇苷、新异落新妇苷、异落新妇苷、engeletin、isoengeletin、spinosin、蛇床子苷A、rutaevin和柠檬苦素。还在体外分析了鉴定的组分的作用。结果显示，与脂多糖（LPS）处理的细胞相比，棘球蛋白和蜂毒苷处理的RAW 264.7细胞的IL-1 β表达以及野牡丹苷D和蜂毒苷处理的RAW 264.7细胞的TNF-α水平没有显著差异。虽然所有潜在活性成分对HaCaT细胞均无凋亡作用，但与对照组相比，三七皂苷D、三七皂苷A、落新妇苷和新异落新妇苷能显著抑制HUVEC细胞的迁移。这些结果表明，靶细胞钓取结合LC-MS分析是从复杂的CMF中筛选活性成分的有效工具，所鉴定的活性成分可能是FZHFZY治疗作用的主要贡献者。(C)2020爱思唯尔B. V.保留所有权利。
Chinese medicine formula (CMF) usually contains multiple herbs and its components are very complicated, which therapeutic effects are derived from the integrative activity of multiple components. Therefore, the screening of bioactive compounds in the formula is necessary, though it is very difficult, not only for elucidating the therapeutic components but also for developing the quality control method. In this work, three cell lines fishing combined with LC-MS analysis was developed for screening the potential bioactive compounds in Fuzhengzhiyanghefuzhiyang decoction (FZHFZY), a CMF for the treatment of psoriasis in clinic. Nineteen potential active compounds were discovered and thirteen of them were identified as rehmannioside D, melittoside or rehmannioside A, tufulingoside, neoastilbin, astilbin, neoisoastilbin, isoastilbin, engeletin, isoengeletin, spinosin, cnidimoside A, rutaevin and limonin, respectively. The effects of identified components were also analyzed in vitro. The results showed that IL-1 beta expression of RAW 264.7 cells treated with spinosin and melittoside as well as the TNF-alpha level of RAW 264.7 cells treated with rehmannioside D and melittoside were not significantly different compared with lipopolysaccharide (LPS)-treated cells. Though all identified potential active components had no apoptosis effect to HaCaT cells, rehmannioside D, rehmannioside A, astilbin and neoisoastilbin could significantly suppress HUVEC cells migration compared with control. These results indicated that the target cell fishing combined with LC-MS analysis is a useful tool for screening bioactive compounds from complicated CMF, and the identified active components may mainly contribute to the therapeutic effects of FZHFZY. (C) 2020 Elsevier B.V. All rights reserved.