Respiratory outcomes after mid-cervical transplantation of embryonic medullary cells in rats with cervical spinal cord injury.
Respiratory outcomes after mid-cervical transplantation of embryonic medullary cells in rats with cervical spinal cord injury.
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DOI：
10.1016/j.expneurol.2016.01.017
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发表时间：
2016-04
影响因子：
5.3
通讯作者：
Fuller DD
中科院分区：
文献类型：
--
作者：
Dougherty BJ;Gonzalez-Rothi EJ;Lee KZ;Ross HH;Reier PJ;Fuller DD
脊髓5-羟色胺对颈髓损伤后呼吸运动输出有很大影响。我们假设，脊髓内移植胚胎中线脑干（MB）细胞丰富的肾上腺素能中缝神经元将改善脊髓损伤后的呼吸功能。在第二颈段（C2 Hx）半切后一周，将胚胎（E14）MB细胞、胎儿脊髓细胞（FSC）或仅培养基（假手术）的悬浮液递送至成年雄性大鼠的背侧C3脊髓。六周后，使用体积描记法评估通气;在一个大鼠亚组中评估膈神经活动。12只接受MB衍生移植物的大鼠中有7只在C3-4背侧白色物质中有明确的组织学证据显示降钙素阳性神经元。移植对基线呼吸模式没有影响，但在短暂的呼吸挑战（7%吸入CO2）期间，与MB移植失败、FSC或假移植的大鼠相比，MB移植成功的大鼠通气量增加。记录从膈神经同侧C2 Hx也表明增加输出在呼吸挑战与成功的MB移植大鼠。我们的结论是，脊髓内同种异体移植E14 MB细胞可以有积极的影响，呼吸运动恢复高度脊髓损伤后。
Respiratory motor output after cervical spinal cord injury (cSCI) is profoundly influenced by spinal serotonin. We hypothesized that intraspinal transplantation of embryonic midline brainstem (MB) cells rich in serotonergic raphé neurons would improve respiratory outcomes after cSCI. One week after hemisection of the 2nd cervical segment (C2Hx) a suspension of either embryonic (E14) MB cells, fetal spinal cord cells (FSC), or media only (sham) was delivered to the dorsal C3 spinal cord of adult male rats. Six weeks later, ventilation was evaluated using plethysmography; phrenic nerve activity was evaluated in a subset of rats. Seven of 12 rats receiving MB-derived grafts had clear histological evidence of serotonin-positive neurons in the C3-4 dorsal white matter. The transplantations had no impact on baseline breathing patterns, but during a brief respiratory challenge (7% inspired CO2) rats with successful MB grafts had increased ventilation compared to rats with failed MB grafts, FSC or sham grafts. Recordings from the phrenic nerve ipsilateral to C2Hx also indicated increased output during respiratory challenge in rats with successful MB grafts. We conclude that intraspinal allografting of E14 MB cells can have a positive impact on respiratory motor recovery following high cSCI.