HIGH-FREQUENCY TRANSFECTION OF CHO CELLS USING POLYBRENE
HIGH-FREQUENCY TRANSFECTION OF CHO CELLS USING POLYBRENE
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DOI：
10.1007/bf01570782
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发表时间：
1986-05-01
期刊：
影响因子：
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通讯作者：
STANLEY, P
中科院分区：
文献类型：
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作者：
CHANEY, WG;HOWARD, DR;STANLEY, P
使用聚凝胺和二甲基亚砜，已实现了几种质粒、粘粒文库和基因组DNA对CHO细胞的高频转染。检测的所有质粒转染子均稳定，并在基因组DNA中显示质粒序列。该方法简单，重现性好，在存在或不存在载体DNA的情况下，即使在非常低浓度的质粒DNA下，也能成功地用几个独立的CHO克隆进行检测。
High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.