Molecular heterogeneity of the bullous pemphigoid antigens as detected by immunoblotting.
Molecular heterogeneity of the bullous pemphigoid antigens as detected by immunoblotting.
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DOI：
10.4049/jimmunol.136.4.1231
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发表时间：
1985-06
影响因子：
4.4
通讯作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz
中科院分区：
文献类型：
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作者：
Ramzy S. Labib;G. Anhalt;Harikrishna Patel;D. Mutasim;L. Diaz
用免疫印迹技术检测了28例大疱性类天疱疮（BP）、4例瘢痕性类天疱疮（CP）和24例对照（正常志愿者和天疱疮、系统性红斑狼疮或其他皮肤病患者）的血清对人表皮提取物的抗作用。提取缓冲液包括1%SDS、5% β-巯基乙醇和6种具有不同特异性的蛋白酶抑制剂。来自个体患者的BP血清与相同的表皮提取物显示出不同的反应模式，并且每个模式由一个或多个条带组成。共5条240 kD、200 kD、180 kD、97 kD和77 kD的条带与BP血清反应; 240 kD的条带与CP血清反应，而对照血清未检测到这些条带。240-kD和180-kD条带与一些血清反应非常强烈，最常观察到（分别为43%和29%）。200-kD、97-kD和77-kD条带较少观察到（分别为25%、7%和7%），但当存在时，其反应通常较强。11%的BP血清不与任何条带反应。与以前的报道相反，这项研究表明，BP自身抗体与几个蛋白质条带反应，通过免疫印迹检测。我们最近通过免疫电镜显示BP自身抗体与基底细胞半桥粒结合。这些蛋白质条带中哪些代表特异性半桥粒蛋白，哪些抗体-抗原相互作用与该疾病的发病机制相关，仍有待确定。
Sera from 28 patients with bullous pemphigoid (BP), four patients with cicatricial pemphigoid (CP), and 24 controls (normal volunteers and patients with pemphigus, systemic lupus erythematosus, or other skin diseases) were tested against extracts of human epidermis by immunoblotting techniques. The extraction buffer included 1% SDS, 5% beta-mercaptoethanol, and six protease inhibitors with various specificities. BP sera from individual patients showed different patterns of reactivity with the same epidermal extract, and each pattern consisted of one or more bands. A total of five bands of 240 kD, 200 kD, 180 kD, 97 kD, and 77 kD reacted with BP sera; the 240-kD band reacted with one CP sera, and none of these bands was detected by the control sera. The 240-kD and 180-kD bands reacted very strongly with some sera and were most frequently observed (43% and 29%, respectively). The 200-kD, 97-kD, and 77-kD bands were less frequently observed (25%, 7%, and 7%, respectively), but when present, their reactions were usually strong. Eleven percent of the BP sera did not react with any bands. Contrary to previous reports, this study shows that BP autoantibodies react with several protein bands, as detected by immunoblotting. We have recently shown by immunoelectron microscopy that BP autoantibodies bind to the basal cell hemidesmosomes. It remains to be determined which of these protein bands represent specific hemidesmosomal proteins and which antibody-antigen interactions are relevant to the pathogenesis of this disease.