Photoreceptor repair in response to RPE transplants in RCS rats: Outer segment regeneration
Photoreceptor repair in response to RPE transplants in RCS rats: Outer segment regeneration
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DOI：
10.3109/02713689609017657
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发表时间：
1996-10-01
影响因子：
2
通讯作者：
Turner, JE
中科院分区：
文献类型：
--
作者：
Lin, N;Fan, W;Turner, JE
目的.我们先前已经证明，正常大鼠新生RPE细胞移植挽救了皇家学院（RCS）营养不良大鼠视网膜中的感光细胞长达一年。在这项研究中，我们研究了RCS大鼠的光感受器救援效果在移植后的前三周内，试图确定RPE移植是否启动修复机制，特别是外节（OS）再生。将新生有色Long Evans大鼠新鲜分离的RPE细胞经巩膜移植到22-23日龄RCS大鼠视网膜下腔。对于对照组，同样注射溶剂。当在移植后10天分析时，在RPE细胞移植物的区域中观察到长的内节和外节生长的短芽。在第10天时，外节的长度不足以测量，但到第14天和第21天时，OS分别为2.02 +/- 0.32 μ m和18.80 +/- 2.78 μ m。在手术后10至21天的溶剂注射视网膜中，未观察到外段，内段比RPE移植视网膜短三倍。在移植后10天，大多数RPE细胞可见于视网膜下腔，但少数已附着于Bruch膜;然而，到21天，许多移植的RPE细胞已附着于Bruch膜，但少数游离于视网膜下腔。这项研究表明，正常大鼠新生RPE细胞的移植不仅有能力支持感光细胞存活，而且还启动早期修复机制，如RCS视网膜外段再生所示。这些结果也决定性地显示了RPE在外节生长和成熟中起重要作用。
Purpose. We have previously shown that transplants of normal rat neonatal RPE cells rescued photoreceptor cells in retinas of Royal College of Surgeons (RCS) dystrophic rats for up to one year. In this study, we investigated the photoreceptor rescue effects in RCS rats within the first three weeks following transplantation in an attempt to determine if RPE transplants initiate repair mechanisms, specifically outer segment (OS) regeneration.Methods. Freshly isolated RPE cells from neonatal pigmented Long Evans rats were transplanted into the subretinal space of 22-23 day-old RCS rats using a transscleral approach. For controls, vehicle was similarly injected.Results. When analyzed at 10 days post-transplantation, long inner segments were observed with short buds of outer segment growth in the area of the RPE-cell transplants. The outer segments were of insufficient length to be measured at 10 days, but by 14 and 21 days, OS were 2.02 +/- 0.32 mu m and 18.80 +/- 2.78 mu m, respectively. In vehicle-injected retinas from 10 to 21 days postsurgery, outer segments were not observed and the inner segments were three-fold shorter than in RPE-transplanted retinas. At 10 days post-transplantation, most RPE cells were seen in the subretinal space, but a few had attached to Bruch's membrane; however, by 21 days, many of the transplanted RPE cells had attached to Bruchs membrane, although a few were found free in the subretinal space.Conclusions. This study has shown that transplants of normal rat neonatal RPE cells have the capacity to support not only photoreceptor cell survival but also initiate early repair mechanisms as exhibited by outer segment regeneration in RCS retinas. These results also conclusively show the important role that the RPE plays in outer segment growth and maturation.