PROINFLAMMATORY CYTOKINES IN NASAL SECRETIONS OF ALLERGIC SUBJECTS AFTER ANTIGEN CHALLENGE
PROINFLAMMATORY CYTOKINES IN NASAL SECRETIONS OF ALLERGIC SUBJECTS AFTER ANTIGEN CHALLENGE
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DOI：
10.1164/ajrccm.149.2.8306027
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发表时间：
1994-02-01
影响因子：
24.7
通讯作者：
ALAM, R
中科院分区：
文献类型：
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作者：
SIM, TC;GRANT, JA;ALAM, R
为了研究细胞因子在变态反应晚期反应（LPR）中的作用，我们测定了8名过敏受试者在抗原或生理盐水激发后鼻分泌物（NS）中的细胞因子（白细胞介素[IL]-1 β、IL-2、IL-4、IL-5、IL-6和粒细胞-巨噬细胞集落刺激因子[GM-CSF]）。通过新开发的基质方法，在激发后每小时收集NS 10 h。所有受试者记录每小时症状评分。使用特异性酶联免疫吸附试验（ELISA）测定细胞因子。与攻击前的值相比，在抗原攻击后的即刻反应（峰值，51.0 +/- 22.4 pg/ml）和LPR（峰值，78.5 +/- 22.6 pg/ml）期间（p < 0.01），而不是盐水攻击，在所有受试者中检测到显著水平的IL-1 β。相反，GM-CSF和IL-6在抗原激发期的第4小时显示出延迟的升高（峰值，分别为26.4 +/-1.3pg/ml和33.8a10.0pg/ml）（p < 0.01，相对于盐水）。来自4个供体的NS也显示出可检测的IL-5（7.6至155 pg/ml），其在立即反应期间和过敏原攻击后的LPR（相对于盐水，p < 0.01）。细胞因子水平与即刻反应（IL-1 β）和LPR（IL-1 β、GM-CSF和IL-6）期间相应的总症状评分相关（p < 0.05）。在所有样本中均未检测到IL-2和IL-4。因此，IL-1 β、IL-5、IL-6和GM-CSF存在于变应性鼻炎的LPR中，它们与临床反应的相关性可能表明它们在变应性炎症中的作用。
To study the role of cytokines in allergic late-phase reactions (LPR), we measured cytokines (interleukins [IL]-1 beta, IL-2, IL-4, IL-5, IL-6, and granulocyte-macrophage colony-stimulating factor [GM-CSF]) in nasal secretions (NS) of eight allergic subjects following antigen or saline provocation. NS were collected hourly for 10 h after challenge by a newly developed matrix method. All subjects recorded hourly symptom scores. Cytokines were measured using specific enzyme-linked immunosorbent assays (ELISA). Compared with prechallenge values, significant levels of IL-1 beta were detected in all subjects during the immediate reaction (peak, 51.0 +/- 22.4 pg/ml) and LPR (peak, 78.5 +/- 22.6 pg/ml) after antigen challenges (p < 0.01) but not saline challenges. In contrast, GM-CSF and IL-6 showed a delayed rise (peak, 26.4 +/- 1.3 pg/ml and 33.8 a 10.0 pg/ml, respectively) at hour 4 in the antigen-challenge period (p < 0.01 versus saline). NS from 4 donors also showed detectable IL-5 (7.6 to 155 pg/ml) during the immediate reaction and LPR after allergen challenges (versus saline, p < 0.01). The levels of cytokine correlated (p < 0.05) with corresponding total symptom scores during the immediate reaction (IL-1 beta) and LPR (IL-1 beta, GM-CSF, and IL-6). IL-2 and IL-4 were not detected in any sample. Thus, IL-1 beta, IL-5, IL-6, and GM-CSF are present in the LPR of allergic rhinitis, and their correlation with clinical responses may suggest their role in allergic inflammation.