Sequences of the 5' portion of the human c-sis gene: characterization of the transcriptional promoter and regulation of expression of the protein product by 5' untranslated mRNA sequences.
Sequences of the 5' portion of the human c-sis gene: characterization of the transcriptional promoter and regulation of expression of the protein product by 5' untranslated mRNA sequences.
复制标题
人类 c-sis 基因 5 部分的序列：转录启动子的表征以及 5 非翻译 mRNA 序列对蛋白质产物表达的调节。
DOI：
10.1093/nar/15.15.6017
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发表时间：
1987
影响因子：
14.9
通讯作者：
Collins,T
中科院分区：
文献类型：
--
作者：
Ratner,L;Thielan,B;Collins,T
该基因编码血小板源性生长因子（PDGF）的B多肽链，在许多正常和病理状态下表达。为了研究人源性蛋白质合成的控制，我们对该基因座上调节转录和翻译的两个区域进行了研究。获得该基因5 ′部分的克隆，其包括RNA起始位点上游的1361个核苷酸。该区域的转录启动子活性在正常和转化细胞中使用质粒与融合到指示基因氯霉素乙酰转移酶的thec-sisRNA起始位点上游的序列进行了证明。还进行了实验，以确定其他可能的调控区域的sisgene。这些数据表明，编码thec-sismRNA的5'非翻译区的thec-sis 1外显子的一部分在体外抑制PDGF B的合成。这些结果确定了在正常条件下转录和翻译的调节以及在几种人类疾病的发病机制中可能起重要作用的sisgene区域。
Thec-sisgene encodes the B polypeptide chain of plateletderived growth factor (PDGF), and is expressed in a number of normal and pathological conditions. In order to study the control of synthesis of the humanc-sisproduct, we have initiated a study of two regions of this genetic locus which regulate transcription and translation. A clone of the 5' portion of the gene was obtained which included 1361 nucleotides upstream of the RNA initiation site. Transcriptional promoter activity of this region was demonstrated in normal and transformed cells using a plasmid with the sequences upstream of thec-sisRNA initiation site fused to an indicator gene, chloramphenicol acetyl transferase. Experiments were also performed to identify other possible regulatory regions of thec-sisgene. These data demonstrated that a portion of thec-sisfirst exon encoding the 5' untranslated region of thec-sismRNA inhibited synthesis of the PDGF B productin vitro. These results define regions of thec-sisgene whose activity may be important in the regulation of transcription and translation under normal conditions and in the pathogenesis several human diseases.