Molecular cloning and expression of the novel splice variants of K(+) channel-interacting protein 2.
Molecular cloning and expression of the novel splice variants of K(+) channel-interacting protein 2.
复制标题
K( ) 通道相互作用蛋白 2 新型剪接变体的分子克隆和表达。
DOI：
10.1006/bbrc.2001.4558
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发表时间：
2001
影响因子：
3.1
通讯作者：
Y. Imaizumi
中科院分区：
文献类型：
--
作者：
S. Ohya;Y. Morohashi;K. Muraki;T. Tomita;M. Watanabe;T. Iwatsubo;Y. Imaizumi
最近报道的两种编码K（+）通道相互作用蛋白2（KChIP 2）剪接变体的cDNA被称为人KChIP 2，已通过RT-PCR从大鼠、小鼠和人心脏中鉴定出来。较长的变体KChIP 2L编码270个氨基酸的蛋白质，与较短的变体KChIP 2S相比，其N末端插入了50个氨基酸。有趣的是，KChIP 2S和KChIP 2L（KChIP 2S/L），而不是原来的KChIP 2在人心脏和脐静脉内皮细胞（HUVECs）中表达。KChIP 2S转录本而不是KChIP 2L主要在大鼠、小鼠和人类心脏和HUVEC中表达，而这两种转录本在其他组织如脑、主动脉和肾脏中以低水平表达。将绿色荧光蛋白（GFP）与KChIP 2S/L的N端融合，构建了Kv4.3与KChIP 2S/L的嵌合蛋白，并在HEK 293细胞中进行了分析。仅在Kv4.3表达的HEK 293细胞中观察到GFP融合的KChIP 2S/L蛋白在细胞膜上或附近的特异性定位。
Two cDNAs encoding the splice variants of K(+) channel-interacting protein 2 (KChIP2) recently reported as human KChIP2 have been identified from rat, mouse, and human heart by RT-PCR. A longer variant, KChIP2L encodes a protein of 270 amino acids, which has a 50-amino-acid insertion in N-terminus in comparison with a shorter one, KChIP2S. Interestingly, both KChIP2S and KChIP2L (KChIP2S/L) but not the original KChIP2 were expressed in human heart and umbilical vein endothelial cells (HUVECs). KChIP2S transcripts but not KChIP2L were predominantly expressed in rat, mouse, and human heart and HUVECs, whereas both transcripts were expressed at low levels in other tissues such as brain, aorta, and kidney. Using chimeric proteins of green fluorescence protein (GFP) fused to the N-terminus of KChIP2S/L, the interactions between Kv4.3 and KChIP2S/L were analyzed in native and Kv4.3-expressed HEK293 cells. Specific localization of GFP-fused KChIP2S/L proteins on or near cell membrane was observed only in Kv4.3-expressed HEK293 cells.
DOI：
10.1073/pnas.93.17.9200
发表时间：
1996-08-20
影响因子：
11.1
作者：
TsengCrank, J;Godinot, N;Reinhart, PH
通讯作者：
Reinhart, PH
年轻和成人心房肌细胞中 Ito 的比较：发育变化的证据。
DOI：
10.1152/ajpheart.1995.268.3.h1335
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
CrumbJr,WJ;Pigott,JD;Clarkson,CW
通讯作者：
Clarkson,CW
影响因子：
37.8
作者：
Kääb, S;Dixon, J;Tomaselli, GF
通讯作者：
Tomaselli, GF