Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump
Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump
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DOI：
10.1073/pnas.191389398
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发表时间：
2001-09-25
影响因子：
11.1
通讯作者：
Fink, GR
中科院分区：
文献类型：
--
作者：
Gaxiola, RA;Li, JS;Fink, GR
过表达液泡H+-焦磷酸酶的转基因植物比同基因野生型菌株更能抵抗高浓度的NaCl和水分剥夺。这些转基因植物比野生型在其叶组织中积累更多的Na+和K+。此外，对来自AVP 1转基因植物和野生型的分离的液泡膜囊泡的直接测量表明，来自转基因植物的囊泡具有增强的阳离子吸收。AVP 1转基因植株的表型表明，增加液泡质子梯度的结果增加溶质积累和水分保持。据推测，阳离子在液泡中的隔离减少了它们的毒性作用。基因工程耐旱耐盐植物可以为开垦因盐碱和降雨量不足而丧失的农田提供一条途径。
Transgenic plants overexpressing the vacuolar H+-pyrophosphatase are much more resistant to high concentrations of NaCl and to water deprivation than the isogenic wild-type strains. These transgenic plants accumulate more Na+ and K+ in their leaf tissue than the wild type. Moreover, direct measurements on isolated vacuolar membrane vesicles derived from the AVP1 transgenic plants and from wild type demonstrate that the vesicles from the transgenic plants have enhanced cation uptake. The phenotypes of the AVP1 transgenic plants suggest that increasing the vacuolar proton gradient results in increased solute accumulation and water retention. Presumably, sequestration of cations in the vacuole reduces their toxic effects. Genetically engineered drought- and salt-tolerant plants could provide an avenue to the reclamation of farmlands lost to agriculture because of salinity and a lack of rainfall.