Effect of glycerol treatment on the calcium current of frog skeletal muscle.
Effect of glycerol treatment on the calcium current of frog skeletal muscle.
复制标题
甘油处理对青蛙骨骼肌钙电流的影响。
DOI：
10.1113/jphysiol.1980.sp013351
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
E. Stefani
中科院分区：
文献类型：
--
作者：
L. N. Siri;J. A. Sánchez;E. Stefani
1.对蛙骨骼肌纤维进行了电流钳和电压钳实验。对于电压钳，使用靠近纤维末端的三个微电极技术。2.在TEA硫酸盐盐水中记录钙峰和电流。添加400 mM甘油不会明显改变它们。3.用甘油法使肌纤维去微管。他们显示钠传播动作电位，钠和钾电流的预期幅度。4.钙峰和电流减少或取消在去管肌纤维。5.纤维电容的分析表明，剩余的伊卡和横向管状系统和表面膜之间的电不连续性的程度之间的线性相关性。6.这些结果表明，伊卡主要位于横管系统。这种定位与机械激活期间的某些作用相容。
1. Current and voltage clamp experiments were carried out on frog skeletal muscle fibres. For voltage clamp, the three micro‐electrode technique near the fibre end was used. 2. Calcium spikes and currents were recorded in TEA sulphate saline. The addition of 400 mM‐glycerol did not appreciably modify them. 3. Muscle fibers were detubulated with the glycerol method. They showed sodium propagating action potentials, with sodium and potassium currents of expected amplitudes. 4. Calcium spikes and currents were reduced or abolished in detubulated muscle fibres. 5. An analysis of fibre capacitance showed a linear correlation between the remaining ICa and the degree of electric discontinuity between the transverse tubular system and the surface membrane. 6. These results indicate that ICa is mainly located in the transverse tubular system. This localization is compatible with some role during mechanical activation.