Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay
Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay
复制标题
DOI：
10.2323/jgam.56.187
复制
发表时间：
2010-06-01
影响因子：
1.2
通讯作者：
Sekine, Yasuhiko
中科院分区：
文献类型：
--
作者：
Saito, Takashi;Chibazakura, Taku;Sekine, Yasuhiko
转座子在细菌基因组的进化中起着重要的作用。量化由转座子引起的转座事件的频率将有助于理解其作用。在这里，我们报告成功的测量IS 1转座的频率使用“GFP跳上测定”，其中转座依赖的GFP表达由流式细胞仪监测。该测定允许在单细胞规模上容易地评估IS转座到染色体DNA中;这是优于测量转座频率的其他常规方法的优点。
Transposons play a significant role in the evolution of bacterial genomes. Quantifying frequency of transpositional events caused by a transposon will facilitate understanding its role. Here, we report successful measurement of the frequency of IS1 transposition using "GFP hop-on assay" in which transposition-dependent GFP expression is monitored by FACS. This assay allows easy assessment of IS transposition into the chromosomal DNA on a single-cell scale; this is an advantage over other conventional methods to measure transposition frequency.