MUTANT FREQUENCY AT THE HPRT LOCUS IN PERIPHERAL-BLOOD T-LYMPHOCYTES OF ATOMIC-BOMB SURVIVORS
MUTANT FREQUENCY AT THE HPRT LOCUS IN PERIPHERAL-BLOOD T-LYMPHOCYTES OF ATOMIC-BOMB SURVIVORS
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DOI：
10.1016/0027-5107(95)00044-j
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发表时间：
1995-07-01
影响因子：
2.3
通讯作者：
AKIYAMA, M
中科院分区：
文献类型：
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作者：
HIRAI, Y;KUSUNOKI, Y;AKIYAMA, M
用重组人白介素2克隆试验检测了254例原子弹幸存者(171例暴露于原子弹，83例对照)外周血淋巴细胞次黄嘌呤-鸟嘌呤磷酸核糖转移酶基因突变频率。在检查时，对原子弹辐射剂量和吸烟状况有微弱但显着的影响，但对年龄和性别没有影响。然而，剂量-反应曲线的斜率相当小，如果不包括来自三个具有极高突变频率的个人的数据，吸烟效应不会显著。剂量反应的疲软至少部分是由于辐射暴露后50年的时间推移。在254例幸存者中，23例有淋巴细胞染色体畸变率，且剂量效应非常显著。但突变频率与细胞畸变率之间的相关性不显著。结论是，淋巴细胞突变试验目前还不够灵敏，不能用于辐射暴露幸存者的生物剂量测定。
The mutant frequency at the hypoxanthine-guanine phosphoribosyltransferase locus in peripheral blood lymphocytes was measured for 254 atomic bomb survivors (171 exposed and 83 control survivors) by a colony assay using recombinant human interleukin-2. Weak but significant effects were detected for atomic bomb radiation dose and smoking status at the time of examination but not for age and sex. However, the slope of the dose-response curve is quite small, and the smoking effect would not have been significant without the inclusion of data from just three individuals with extremely high mutant frequencies. The weakness of the dose response is at least partly due to the time lapse of 50 years since radiation exposure. Among the 254 survivors, 23 had chromosome aberration data in lymphocytes and the dose response was highly significant. However, the correlation between the mutant frequency and the proportion of cells with aberrations was not significant. It was concluded that the lymphocyte mutation assay is presently not sensitive enough for biological dosimetry of radiation exposure in the survivors.