Effect of arbuscular mycorrhizal fungi inoculation on cold stress-induced oxidative damage in leaves of Elymus nutans Griseb
Effect of arbuscular mycorrhizal fungi inoculation on cold stress-induced oxidative damage in leaves of Elymus nutans Griseb
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DOI：
10.1016/j.sajb.2015.10.001
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发表时间：
2016-05-01
影响因子：
3.1
通讯作者：
Hu, T. M.
中科院分区：
文献类型：
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作者：
Chu, X. T.;Fu, J. J.;Hu, T. M.
为了研究丛枝菌根真菌（AMF）接种和短期冷暴露对羊草（Elymus nutans Griseb）的影响，以郑岛（ZD）和康马（KM）两个品种45 d龄幼苗接种苔藓球囊菌（Glomus mosseae）后进行5 d的冷处理。测定了AMF对两个品种生理机能的影响，尤其对冷胁迫下的氧化指标感兴趣。在低温胁迫下，植物体内活性氧（ROS）（包括超氧化物和过氧化氢）的积累量非常高，导致细胞膜脂质过氧化，主要表现为相对电解质泄漏（REL）和丙二醛（MDA）含量。AMF定殖植株的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）活性较高，其氧化损伤较小。菌根接种能促进植株生长，提高叶绿素含量和谷胱甘肽、可溶性糖等抗氧化物质含量。我们还测量了菌根定植的程度。这些保护机制在耐温型KM中比在温度敏感型ZD中更有效。综上所述，接种AMF可以通过直接清除活性氧和调节氧化还原平衡等防御机制提高植物对冷胁迫的抗性。(c) 2016年萨博。Elsevier B.V.版权所有。
To investigate the effect of arbuscular mycorrhizal fungi (AMF) inoculation and short-term cold exposer on Elymus nutans Griseb, 45 day old seedlings of two cultivars, Zhengdao (ZD) and Kangma (KM), were subjected to a 5 day cold treatment after inoculated with Glomus mosseae. The effects of AMF on the physiology of the two cultivars were determined, and we were especially interested in oxidative indexes under cold stress. Accumulation of reactive oxygen species (ROS), including superoxide and hydrogen peroxide, was very high in cold-stressed plants and caused lipid peroxidation in membranes, which measured as relative electrolyte leakage (REL), malondialdehyde (MDA) content. Less oxidative damage was detected in AMF colonized plants, which was associated with higher activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). Mycorrhizal inoculum application promoted plant growth and enhanced the level of chlorophyll and antioxidant compounds such as glutathione and soluble sugars. We also measured the extent of mycorrhizal colonization. These protective mechanisms were found to be more efficient in temperature-tolerant KM than temperature-sensitive ZD. Overall, we suggest that AMF inoculation can improve plant resistance to cold stress in E. nuatans seedlings by directly scavenging ROS and by modulating redox balance and other defense mechanisms. (C) 2016 SAAB. Published by Elsevier B.V. All rights reserved.