Leptin immunoreactivity in the central nervous system in normal and diabetic rats
Leptin immunoreactivity in the central nervous system in normal and diabetic rats
复制标题
DOI：
10.1097/00001756-199902050-00042
复制
发表时间：
1999-02-05
期刊：
影响因子：
1.7
通讯作者：
Yeh, RS
中科院分区：
文献类型：
--
作者：
Li, HY;Wang, LL;Yeh, RS
使用抗瘦素多克隆抗血清检测大鼠中枢神经系统的瘦素免疫反应性（-ir）。瘦素-ir被发现存在于下丘脑的核周和神经元突起中，包括室旁核，以及脑干的几个区域，包括孤束核。在链脲佐菌素治疗的糖尿病大鼠中，瘦素-ir仅在下丘脑中显着减弱，并通过胰岛素治疗恢复。我们的研究结果表明，全身胰岛素的去除或恢复可调节下丘脑瘦素-ir，这表明在大脑中观察到的瘦素-ir对外周激素状态敏感。下丘脑中胰岛素调节的瘦素的存在揭示了瘦素在调节能量平衡和代谢中的中心作用的新研究途径。 (C) 1999 年利平科特·威廉姆斯和威尔金斯。
AN anti-leptin polyclonal antiserum was used to detect leptin-immunoreactivity (-ir) in the central nervous system in rats. Leptin-ir was found in perikaryon and processes of neurons in the hypothalamus, including the paraventricular nucleus, and in several regions in the brainstem, including the nucleus of the solitary tract. In streptozotocin-treated diabetic rats, leptin-ir was dramatically attenuated only in the hypothalamus, and was restored by insulin treatment. Our findings that removal or restoration of systemic insulin regulates hypothalamic leptin-ir suggests that the leptin-ir observed in the brain is sensitive to peripheral hormone status. The presence of insulin-regulated leptin in the hypothalamus reveals a new avenue of research on the central actions of leptin in regulating energy balance and metabolism. (C) 1999 Lippincott Williams & Wilkins.