Measurement of cerebral blood volume in mouse brain regions using micro-computed tomography
Measurement of cerebral blood volume in mouse brain regions using micro-computed tomography
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DOI：
10.1016/j.neuroimage.2009.03.083
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发表时间：
2009-10-01
期刊：
影响因子：
5.7
通讯作者：
Sled, John G.
中科院分区：
文献类型：
--
作者：
Chugh, Brige P.;Lerch, Jason P.;Sled, John G.
微计算机断层扫描（micro-CT）是一种X射线成像技术，可以产生详细的脑血管三维图像。本文介绍了一种新的方法，使用micro-CT测量脑血容量（CBV）在小鼠大脑的发展。作为应用的方法，我们测试的假设，CBV存在差异的解剖大脑区域和高能量要求的初级感觉皮层区域局部升高CBV，这可能反映了血管专业化。CBV测量为填充脉管系统的不透射线硅橡胶占据的组织空间的百分比。为了确保CBV测量的准确性，对标准微CT标本制备和分析程序进行了一些创新性的改进。所描述的方法的关键特征是在受控压力下的血管灌注、微CT图像与MRI解剖脑图谱的配准以及微CT强度与CBV单位的重新缩放，其中可选择地排除主要血管。血管灌注的组织学验证表明，血管填充的平均百分比为93 +/-3%。比较9只小鼠的13个脑区，发现不同脑区的CBV存在显著差异（p
Micro-computed tomography (micro-CT) is an X-ray imaging technique that can produce detailed 3D images of cerebral vasculature. This paper describes the development of a novel method for using micro-CT to measure cerebral blood volume (CBV) in the mouse brain. As an application of the methodology, we test the hypotheses that differences in CBV exist over anatomical brain regions and that high energy demanding primary sensory regions of the cortex have locally elevated CBV, which may reflect a vascular specialization. CBV was measured as the percentage of tissue space occupied by a radio-opaque silicon rubber that fills the vasculature. To ensure accuracy of the CBV measurements, several innovative refinements were made to standard micro-CT specimen preparation and analysis procedures. Key features of the described method are vascular perfusion under controlled pressure, registration of the micro-CT images to an MRI anatomical brain atlas and re-scaling of micro-CT intensities to CBV units with selectable exclusion of major vessels. Histological validation of the vascular perfusion showed that the average percentage of vessels filled was 93 +/- 3%. Comparison of thirteen brain regions in nine mice revealed significant differences in CBV between regions (p