Changes in polyphenol and sugar concentrations in wild type and genetically modified Nicotiana langsdorffii Weinmann in response to water and heat stress
Changes in polyphenol and sugar concentrations in wild type and genetically modified Nicotiana langsdorffii Weinmann in response to water and heat stress
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DOI：
10.1016/j.plaphy.2015.09.012
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发表时间：
2015-12-01
影响因子：
6.5
通讯作者：
Del Bubba, Massimo
中科院分区：
文献类型：
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作者：
Ancillotti, Claudia;Bogani, Patrizia;Del Bubba, Massimo
在这项研究中，野生型烟草植物通过插入编码糖皮质激素受体的大鼠基因（gr）或 rolC 基因进行遗传转化，并暴露于水和热胁迫下。通过在生长培养基中添加20% PEG 6000诱导水分胁迫15天，并在50℃下热处理2小时，然后进行再生长能力研究。通过测定电解质渗漏、干重生物量产量和水含量来研究植物对胁迫的反应。这些数据根据总多酚、选定的酚类化合物和一些可溶性糖的抗自由基活性和浓度进行评估，作为基因插入和/或胁迫处理引起的代谢变化的生化指标。关于水胁迫，测量的生理参数证明胁迫水平按 rolC < gr < WT 植物的顺序增加（例如，WT 和 gr 样品中电解质渗漏分别增加约 100% 和 50%），并符合生化模式，即包括抗自由基活性和酚类物质的普遍下降，以及糖的增加。就热应激而言，电解质泄漏数据仅与再生能力研究部分一致。事实上，根据后一种评估，gr 是受热休克影响较小的基因型。在这方面，糖，尤其是酚类化合物可以提供热休克治疗的长期影响的信息。 (C) 2015 Elsevier Masson SAS。版权所有。
In this study wild type Nicotiana langsdorffii plants were genetically transformed by the insertion of the rat gene (gr) encoding the glucocorticoid receptor or the rolC gene and exposed to water and heat stress. Water stress was induced for 15 days by adding 20% PEG 6000 in the growth medium, whereas the heat treatment was performed at 50 degrees C for 2 h, after that a re-growing capability study was carried out. The plant response to stress was investigated by determining electrolyte leakage, dry weight biomass production and water content. These data were evaluated in relation to antiradical activity and concentrations of total polyphenols, selected phenolic compounds and some soluble sugars, as biochemical indicators of metabolic changes due to gene insertion and/or stress treatments.As regards the water stress, the measured physiological parameters evidenced an increasing stress level in the order rolC < gr < WT plants (e.g. about 100% and 50% electrolyte leakage increase in WT and gr samples, respectively) and complied with the biochemical pattern, which consisted in a general decrease of antiradical activity and phenolics, together with an increase in sugars.As regard heat stress, electrolyte leakage data were only in partial agreement with the re-growing capability study. In fact, according to this latter evaluation, gr was the genotype less affected by the heat shock. In this regard, sugars and especially phenolic compounds are informative of the long-term effects due to heat shock treatment. (C) 2015 Elsevier Masson SAS. All rights reserved.