Evidence for anaphase pulling forces during C. elegans meiosis.
Evidence for anaphase pulling forces during C. elegans meiosis.
复制标题
DOI：
10.1083/jcb.202005179
复制
发表时间：
2020-12-07
期刊：
影响因子：
--
通讯作者：
McNally FJ
中科院分区：
文献类型：
--
作者：
Danlasky BM;Panzica MT;McNally KP;Vargas E;Bailey C;Li W;Gong T;Fishman ES;Jiang X;McNally FJ
据报道，在C.优雅的Danlasky等人发现，在C.线虫减数分裂后期染色体运动被认为是由微管末端附着在动粒外表面产生的拉力介导的。然而，有人建议，在C.在秀丽线虫雌性减数分裂中，后期由不依赖于着丝粒的推动机制介导，微管仅附着在分离染色体的内表面。我们发现，着丝粒蛋白KNL-1和KNL-3是需要后期前染色体拉伸，这表明在拉力的作用。在没有KNL-1，3的情况下，成对的同源染色体不分离，也不向纺锤体极移动。相反，在后期B纺锤体伸长过程中，每个同源物对与同一纺锤体极一起移动。因此，两团染色质最终位于纺锤体的两极，表现为成功的分裂后期。
Meiotic anaphase has been reported to be mediated by kinetochore-independent pushing forces in C. elegans. Danlasky et al. find that depletion of kinetochore proteins reduces pulling forces and prevents successful separation of homologous chromosomes during C. elegans meiosis. Anaphase chromosome movement is thought to be mediated by pulling forces generated by end-on attachment of microtubules to the outer face of kinetochores. However, it has been suggested that during C. elegans female meiosis, anaphase is mediated by a kinetochore-independent pushing mechanism with microtubules only attached to the inner face of segregating chromosomes. We found that the kinetochore proteins KNL-1 and KNL-3 are required for preanaphase chromosome stretching, suggesting a role in pulling forces. In the absence of KNL-1,3, pairs of homologous chromosomes did not separate and did not move toward a spindle pole. Instead, each homolog pair moved together with the same spindle pole during anaphase B spindle elongation. Two masses of chromatin thus ended up at opposite spindle poles, giving the appearance of successful anaphase.
登录
查看更多内容
影响因子：
9.2
作者：
Kaitna, S;Pasierbek, P;Glotzer, M
通讯作者：
Glotzer, M
影响因子：
7.7
作者：
Cortes, Daniel;McNally, Karen;McNally, Francis J.
通讯作者：
McNally, Francis J.
影响因子：
3.3
作者：
Ellefson, Marina L.;McNally, Francis J.
通讯作者：
McNally, Francis J.
影响因子：
4.6
作者：
Joly, Nicolas;Martino, Lisa;Pintard, Lionel
通讯作者：
Pintard, Lionel
影响因子：
11.8
作者：
Gui, Liming;Homer, Hayden
通讯作者：
Homer, Hayden