Rho1p, a yeast protein at the interface between cell polarization and morphogenesis
Rho1p, a yeast protein at the interface between cell polarization and morphogenesis
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DOI：
10.1126/science.272.5259.277
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发表时间：
1996-04-12
期刊：
影响因子：
56.9
通讯作者：
Cabib, E
中科院分区：
文献类型：
--
作者：
Drgonova, J;Drgon, T;Cabib, E
酵母菌细胞壁主要结构成分β (1- bbbb3)- d -葡聚糖合成酶（也称为1,3- β -葡聚糖合成酶）的催化合成酶需要鸟苷三磷酸（GTP）结合蛋白才能发挥活性。GTP结合蛋白鉴定为Rho1p。rho1突变体在GTP刺激葡聚糖合成酶方面存在缺陷，通过添加纯化或重组Rho1p来纠正这一缺陷。在rho1菌株的纯化制剂中缺失的蛋白被鉴定为Rho1p。Rho1p也调节蛋白激酶C，它控制着丝裂原激活的蛋白激酶级联反应。显性阳性PKC1基因的实验表明Rho1p的两种作用是相互独立的。Rho1p与肌动蛋白斑块在芽出部位的共定位以及Rho1p在细胞壁合成中的作用强调了Rho1p在极化生长和形态发生中的重要性。
The enzyme that catalyzes the synthesis of the major structural component of the yeast cell wall, beta(1-->3)-D-glucan synthase (also known as 1,3-beta-glucan synthase), requires a guanosine triphosphate (GTP) binding protein for activity. The GTP binding protein was identified as Rho1p. The rho1 mutants were defective in GTP stimulation of glucan synthase, and the defect was corrected by addition of purified or recombinant Rho1p. A protein missing in purified preparations from a rho1 strain was identified as Rho1p. Rho1p also regulates protein kinase C, which controls a mitogen-activated protein kinase cascade. Experiments with a dominant positive PKC1 gene showed that the two effects of Rho1p are independent of each other. The colocalization of Rho1p with actin patches at the site of bud emergence and the role of Rho1p in cell wall synthesis emphasize the importance of Rho1p in polarized growth and morphogenesis.