Analysis of Plasmodium falciparum Na+/H+ exchanger (pfnhe1) polymorphisms among imported African malaria parasites isolated in Wuhan, Central China
Analysis of Plasmodium falciparum Na+/H+ exchanger (pfnhe1) polymorphisms among imported African malaria parasites isolated in Wuhan, Central China
复制标题
武汉地区输入性非洲疟原虫恶性疟原虫Na/H交换体(pfnhe1)多态性分析
DOI：
10.1186/s12879-019-3921-7
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发表时间：
2019-04-29
影响因子：
3.7
通讯作者：
Li，Jian
中科院分区：
文献类型：
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作者：
Wu，Kai;Yao，Yi;Li，Jian
研究背景奎宁（Quinine，QN）是治疗疟疾的有效药物。然而，在许多疟疾流行地区，特别是在非洲国家，已经报告了恶性疟原虫对奎宁的耐药性。恶性疟原虫Na+/H+交换器（pfnhe 1）的遗传多态性被认为影响QN易感性。在这里，ms 4760等位基因的pfnhe 1分析输入非洲恶性疟原虫寄生虫分离从返回的旅行者在武汉，中国中部methodsA共204个干血点收集在2011-2016年。结果99.51%（203/204）的PCR产物进行了序列测定，68.63%（140/204）的分离株成功分析了pfnhe 1 ms 4760单倍型。总共鉴定了28个不同的ms 4760等位基因，其含有0至5个DNNND和1至3个NHNDNHNNDDD重复。在等位基因中，ms 4760 -1（22.86%，32/140）、ms 4760 -3（17.86%，25/140）和ms 4760 -7（10.71%，15/140）是最常见的等位基因型。结论本研究为武汉地区恶性疟原虫pfnhe 1 ms 4760基因多态性的初步分析提供了一个新的方法。Pfnhe 1基因可能是评价疟疾流行区和非流行区QNR患病率的一个良好的遗传标记。
BackgroundQuinine (QN) remains an effective drug for malaria treatment. However, quinine resistance (QNR) in Plasmodium falciparum has been reported in many malaria-endemic regions particularly in African countries. Genetic polymorphism of the P. falciparum Na+/H+ exchanger (pfnhe1) is considered to influence QN susceptibility. Here, ms4760 alleles of pfnhe1 were analysed from imported African P. falciparum parasites isolated from returning travellers in Wuhan, Central China.MethodsA total of 204 dried-blood spots were collected during 2011–2016. The polymorphisms of the pfnhe1 gene were determined using nested PCR with DNA sequencing.ResultsSequences were generated for 99.51% (203/204) of the PCR products and 68.63% (140/204) of the isolates were analysed successfully for the pfnhe1 ms4760 haplotypes. In total, 28 distinct ms4760 alleles containing 0 to 5 DNNND and 1 to 3 NHNDNHNNDDD repeats were identified. For the alleles, ms4760–1 (22.86%, 32/140), ms4760–3 (17.86%, 25/140), and ms4760–7 (10.71%, 15/140) were the most prevalent profiles. Furthermore, 5 undescribed ms4760 alleles were reported.ConclusionsThe study offers an initial comprehensive analysis of pfnhe1 ms4760 polymorphisms from imported P. falciparum isolates in Wuhan. Pfnhe1 may constitute a good genetic marker to evaluate the prevalence of QNR in malaria-endemic and non-endemic regions.