3D In Vitro Neuron on a Chip for Probing Calcium Mechanostimulation
3D In Vitro Neuron on a Chip for Probing Calcium Mechanostimulation
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DOI：
10.1002/adbi.202000080
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发表时间：
2020-09-02
影响因子：
4.1
通讯作者：
LeDuc, Philip R.
中科院分区：
文献类型：
--
作者：
Bobo, Justin;Garg, Akash;LeDuc, Philip R.
芯片系统上组织的进化为模拟生理系统的生物功能的响应提供了希望。组织芯片方法的一个重要方向是基于神经元的系统，它可以模拟神经反应，减少体内实验的需要。对于神经研究，最近更多的注意力集中在理解力学上，这是由于创伤性脑损伤（TBI）和疼痛等领域的问题。为了开始解决这些领域，开发了与机械激励测试床（MET）系统相结合的3D神经集成组织芯片（NITC）方法，以向更逼真的生理环境施加外部机械刺激。使用用神经生长因子分化的PC 12细胞，其在受控的3D支架中培养。在3D NITC系统中用Fluo-4-AM标记细胞，以检查它们在与图像捕获同步的机械刺激下的钙响应。了解2D系统之外的机械刺激的神经反应对于神经学研究和未来的个性化策略非常重要。这项工作将在包括组织芯片系统、生物材料和神经力学在内的多种领域产生影响。
The evolution of tissue on a chip systems holds promise for mimicking the response of biological functionality of physiological systems. One important direction for tissue on a chip approaches are neuron-based systems that could mimic neurological responses and lessen the need for in vivo experimentation. For neural research, more attention has been devoted recently to understanding mechanics due to issues in areas such as traumatic brain injury (TBI) and pain, among others. To begin to address these areas, a 3D Nerve Integrated Tissue on a Chip (NITC) approach combined with a Mechanical Excitation Testbed (MET) System is developed to impose external mechanical stimulation toward more realistic physiological environments. PC12 cells differentiated with nerve growth factor, which were cultured in a controlled 3D scaffolds, are used. The cells are labeled in a 3D NITC system with Fluo-4-AM to examine their calcium response under mechanical stimulation synchronized with image capture. Understanding the neural responses to mechanical stimulation beyond 2D systems is very important for neurological studies and future personalized strategies. This work will have implications in a diversity of areas including tissue-on-a-chip systems, biomaterials, and neuromechanics.