Integration of physical, genetic and cytogenetic maps of human chromosome 7: isolation and analysis of yeast artificial chromosome clones for 117 mapped genetic markers.
Integration of physical, genetic and cytogenetic maps of human chromosome 7: isolation and analysis of yeast artificial chromosome clones for 117 mapped genetic markers.
复制标题
人类 7 号染色体的物理、遗传和细胞遗传学图谱的整合：酵母人工染色体克隆的 117 个映射遗传标记的分离和分析。
DOI：
10.1093/hmg/3.3.489
复制
发表时间：
1994
影响因子：
3.5
通讯作者：
Kimura,J
中科院分区：
文献类型：
--
作者：
Green,ED;Idol,JR;Mohr-Tidwell,RM;Braden,VV;Peluso,DC;Fulton,RS;Massa,HF;Magness,CL;Wilson,AM;Kimura,J
人类基因组计划的一个重要目标是为每个人类染色体组装完全集成的物理，遗传和细胞遗传学图谱。为此，我们分离出了酵母人工染色体（YAC）克隆，其中包含119个遗传标记中的117个，这些标记构成了最近构建的人类7号染色体详细遗传图谱。对这些克隆的分析揭示了许多实例，其中相邻的遗传标记已经物理连接，无论是在单个YAC中还是在多YAC重叠群中。目前，117个遗传标记包含在少于80个YAC重叠群中，这些重叠群中的大多数基于相应标记的遗传图谱位置相对于彼此唯一排序。这些YAC和YAC重叠群估计含有约60-85%的来自人7号染色体的DNA。代表36个遗传标记的YAC通过荧光原位杂交（FISH）映射到中期染色体，允许将这些遗传标记分配到沿7号染色体沿着的细胞遗传学条带，并将着丝粒放置在遗传图谱内。总之，这些研究提供了遗传学和细胞遗传学锚定的YAC克隆，覆盖了大部分的7号染色体，这将是有用的基因的定位克隆和作为一个框架，用于组装一个完整的YAC为基础的物理图谱的染色体。
An Important goal of the human genome project is to assemble fully integrated physical, genetic and cytogenetic maps for each human chromosome. Towards that end, we have isolated yeast artificial chromosome (YAC) clones containing 117 of the 119 genetic markers that constitute a recently constructed, detailed genetic map of human chromosome 7. Analysis of these clones reveals numerous examples where adjacent genetic markers have been physically connected, either In Individual YACs or in multi-YAC contigs. At present, the 117 genetic markers are contained In fewer than 80 YAC contigs, with most of these contigs uniquely ordered relative to one another based on the genetic map positions of the corresponding markers. These YAC and YAC contigs are estimated to contain ˜60–85% of the DNA from human chromosome 7. YACs representing 36 genetic markers were mapped by fluorescenceIn situhybridization (FISH) to metaphase chromosomes, allowing assignment of these genetic markers to cytogenetic bands along chromosome 7 and placement of the centromere within the genetic map. Together, these studies provide genetically and cytogenetically anchored YAC clones covering the majority of chromosome 7 that will be useful both for the positional cloning of genes and as a framework for assembling a complete YAC-based physical map of the chromosome.