Spider Silk Capsules as Protective Reaction Containers for Enzymes
Spider Silk Capsules as Protective Reaction Containers for Enzymes
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DOI：
10.1002/adfm.201302100
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发表时间：
2014-02-01
影响因子：
19
通讯作者：
Scheibel, Thomas
中科院分区：
文献类型：
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作者：
Bluem, Claudia;Nichtl, Alfons;Scheibel, Thomas
蜘蛛丝纤维以其高拉伸强度和高弹性而闻名。基于重组生产蜘蛛丝蛋白的可能性，目前蜘蛛丝材料的技术应用是可行的。工程化重组蜘蛛丝蛋白eADF4(C16)是基于ADF4(蜘蛛丝素蛋白)的序列，ADF4是欧洲花园蜘蛛牵引丝的至少三种蛋白质之一。蛋白质eADF4(C16)可以加工成不同的形态。这里，eADF4(C16)的胶囊在油/水界面组装。这些微胶囊具有机械稳定性，可用作酶或化学催化剂等较高分子量化合物的运输系统。此外，它们还可以看作是一个带有半透膜的小型封闭反应室。反应可以通过反应物在丝膜中的扩散来引发。EADF4(C16)胶囊可保护用作模型的酶-半乳糖苷酶免受蛋白质降解。-半乳糖苷酶的功能互补显示了这种蜘蛛丝胶囊内酶的可控激活，突出了其作为反应容器的广泛适用性，例如用于酶。
Spider silk fibres are well known for their high tensile strength in combination with high elasticity. Based on the possibility of recombinant production of spider silk proteins, technical applications of spider silk materials are nowadays feasible. The engineered recombinant spider silk protein eADF4(C16) is based on the sequence of ADF4 (Araneus diadematus fibroin), one out of at least three proteins of the dragline silk of the European garden spider A. diadematus. The protein eADF4(C16) can be processed into different morphologies. Here, capsules of eADF4(C16) are assembled at an oil/water interface. These microcapsules are mechanically stable and can be used as a transport system for higher molecular weight compounds such as enzymes or chemical catalysts. Further, they can be regarded as a small enclosed reaction chamber with a semi-permeable membrane. Reactions can be initiated by diffusion of the reactants through the silk membrane. The eADF4(C16) capsules protect the enzyme -galactosidase, used as model, against proteolysis. Functional -complementation of -galactosidase visualizes the controllable activation of an enzyme within such spider silk capsule, highlighting the broad applicability thereof as reaction containers, e.g., for enzymes.