Development- and activity-dependent regulation of SNAP-25 phosphorylation in rat brain
Development- and activity-dependent regulation of SNAP-25 phosphorylation in rat brain
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DOI：
10.1016/j.neulet.2006.08.055
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发表时间：
2006-10-30
影响因子：
2.5
通讯作者：
Takahashi, Masami
中科院分区：
文献类型：
--
作者：
Kataoka, Masakazu;Kuwahara, Reiko;Takahashi, Masami
突触体相关蛋白25 kDa（SNAP-25）是神经递质释放所必需的SNARE蛋白的一个成员，在PC 12细胞和大鼠脑中以蛋白激酶C依赖的方式在Ser（187）处磷酸化。目前还不清楚SNAP-25的磷酸化在发育过程中是如何被神经元活动调节的。我们使用抗磷酸化SNAP-25抗体研究了大鼠脑中Ser（187）处SNAP-25磷酸化的模式。SNAP-25的表达和磷酸化在出生后早期均显著增加，但其起始时间有很大差异。早在胚胎第18天就检测到SNAP-25的表达，而SNAP-25的磷酸化直到出生后第4天才能检测到。在培养的大鼠海马神经元中也观察到磷酸化的发生延迟。SNAP-25的磷酸化以神经元活性依赖的方式调节，并且在大鼠海马中，通过用红藻氨酸引入癫痫发作而降低。这些结果清楚地表明SNAP-25在Ser（187）处的磷酸化在发育和神经活性依赖性方式中受到调节，并且可能在高级脑功能中发挥重要作用。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Synaptosomal-associated protein of 25 kDa (SNAP-25), a member of the SNARE proteins essential for neurotransmitter release, is phosphorylated at Ser(187) in PC12 cells and in the rat brain in a protein kinase C-dependent manner. It remains unclear how the phosphorylation of SNAP-25 is regulated during development and by neuronal activity. We studied the mode of SNAP-25 phosphorylation at Ser(187) in the rat brain using an anti-phosphorylated SNAP-25 antibody. Both the expression and phosphorylation of SNAP-25 increased remarkably during the early postnatal period, but their onsets were quite different. SNAP-25 expression was detected as early as embryonic Day 18, whereas the phosphorylation of SNAP-25 could not be detected until postnatal Day 4. A delay in the onset of phosphorylation was also observed in cultured rat hippocampal neurons. The phosphorylation of SNAP-25 was regulated in a neuronal activity-dependent manner and, in the rat hippocampus, decreased by introducing seizures with kainic acid. These results clearly indicated that the phosphorylation of SNAP-25 at Ser(187) is regulated in development and neuronal activity-dependent manners, and is likely to play important roles in higher brain functions. (c) 2006 Elsevier Ireland Ltd. All rights reserved.