Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
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DOI：
10.1590/s0074-02762007005000116
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发表时间：
2007-11-01
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影响因子：
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通讯作者：
Trabulsi, Luiz Rachid
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作者：
Bueris, Vanessa;Sircili, Marcelo Palma;Trabulsi, Luiz Rachid
作为病例对照研究的一部分，我们从巴西萨尔瓦多巴伊亚州1,207名患有和不患有急性地方性腹泻的儿童中分离出不同的致泻性(DEC)大肠杆菌病毒型。由于DEC的鉴定不能仅基于生化和培养标准，我们采用了一种多重聚合酶链式反应，该方法是由肠致病性大肠埃希氏菌(EPEC)、志贺毒素产生的大肠杆菌/肠出血性大肠杆菌(STEC/EHEC)、产肠毒素大肠杆菌(ETEQ)和肠聚集性大肠杆菌(EAEC)的5对特异引物组合而成，在一步反应中同时检测这些病原菌。为了区分典型和非典型EPEC菌株，对这些菌株进行了EAF质粒的检测。在全球病例对照研究的这一样本中，腹泻组(1020例)和对照组(187例)中致泻性大肠埃希菌的患病率分别为25.4%(259例)和18.7%(35例)。最常见的病原型是EAEC(10.7%)，其次是非典型EPEC(9.4%)、ETEC(3.7%)和STEC(0.6%)。仅在一个样本中检测到典型的EPEC。所研究的腹泻儿童和非腹泻儿童的致病类型的患病率没有显著差异。
We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEQ and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1, 020 patients) and the control group (18 7 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.