Transcriptional markers of sub-optimal nutrition in developing Apis mellifera nurse workers
Transcriptional markers of sub-optimal nutrition in developing Apis mellifera nurse workers
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DOI：
10.1186/1471-2164-15-134
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发表时间：
2014-02-15
期刊：
影响因子：
4.4
通讯作者：
Anderson, Kirk E.
中科院分区：
文献类型：
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作者：
Corby-Harris, Vanessa;Jones, Beryl M.;Anderson, Kirk E.
背景：蜜蜂（Apis mellifera）作为传粉者，对全球经济和生态系统健康做出了重大贡献。花粉对蜜蜂的饮食至关重要，提供蛋白质，脂质和微量营养素。伴随正常工蚁发育的生理、解剖和行为的巨大变化具有高度的可塑性，最近的研究表明，发育，特别是从保育角色到觅食角色的转变，受到饮食的极大影响。然而，饮食在新羽化的蜜蜂向护士的发育过渡中所起的作用却知之甚少。为了进一步了解蜜蜂营养和饮食在护士发育中的作用，我们使用了3天和8天大工蜂的转录组的高通量筛选，这些工蜂在蜂巢内喂食蜂蜜和储存的花粉（丰富的饮食）或仅喂食蜂蜜（贫乏的饮食）。我们采用了三个因素（年龄，饮食，年龄x饮食）的转录组分析，以确定饮食是否影响护士工作者的生理和是否不良饮食改变了通常与aging.Results相关的发育过程：基因表达的实质性变化发生由于饥饿。饮食引起的基因转录变化发生在年轻的蜜蜂很大程度上是那些发生在老年蜜蜂的一个子集，但某些签名的饥饿只明显8天大的工人。在A.在意大利蜜蜂基因组中，150个转录物在3日龄时由于不良饮食而表现出差异表达，相比之下，17，226个转录物在8日龄时由于不良饮食而表现出差异表达，并且与老年蜜蜂相比，不良饮食导致年轻蜜蜂中基因表达更频繁的下调。此外，伴随着早期成年发育的年龄相关的生理变化由于这些年轻成年蜜蜂的饮食而不同。与那些喂食充足饮食的蜜蜂相比，在喂食不良饮食的发育中蜜蜂中观察到更频繁的基因表达下调。功能分析还表明，在良好喂养的蜜蜂中发生的生理和发育过程与在花粉剥夺的蜜蜂中发生的生理和发育过程有很大不同。我们的数据支持这一假设，不良的饮食导致正常的年龄相关的发展goerry.Conclusion：营养不良的生理和年龄相关的发展的护士工蜂的基因表达的重大后果。当然还需要更多的工作来充分了解饥饿的后果以及该系统中营养和发育的复杂生物学，但本研究中确定的基因为理解不良饮食的后果和减轻群体饥饿的经济成本提供了一个起点。
Background: Honey bees (Apis mellifera) contribute substantially to the worldwide economy and ecosystem health as pollinators. Pollen is essential to the bee's diet, providing protein, lipids, and micronutrients. The dramatic shifts in physiology, anatomy, and behavior that accompany normal worker development are highly plastic and recent work demonstrates that development, particularly the transition from nurse to foraging roles, is greatly impacted by diet. However, the role that diet plays in the developmental transition of newly eclosed bees to nurse workers is poorly understood. To further understand honey bee nutrition and the role of diet in nurse development, we used a high-throughput screen of the transcriptome of 3 day and 8 day old worker bees fed either honey and stored pollen (rich diet) or honey alone (poor diet) within the hive. We employed a three factor (age, diet, age x diet) analysis of the transcriptome to determine whether diet affected nurse worker physiology and whether poor diet altered the developmental processes normally associated with aging.Results: Substantial changes in gene expression occurred due to starvation. Diet-induced changes in gene transcription occurring in younger bees were largely a subset of those occurring in older bees, but certain signatures of starvation were only evident 8 day old workers. Of the 18,542 annotated transcripts in the A. mellifera genome, 150 transcripts exhibited differential expression due to poor diet at 3d of age compared with 17,226 transcripts that differed due to poor diet at 8d of age, and poor diet caused more frequent down-regulation of gene expression in younger bees compared to older bees. In addition, the age-related physiological changes that accompanied early adult development differed due to the diet these young adult bees were fed. More frequent down-regulation of gene expression was observed in developing bees fed a poor diet compared to those fed an adequate diet. Functional analyses also suggest that the physiological and developmental processes occurring in well-fed bees are vastly different than those occurring in pollen deprived bees. Our data support the hypothesis that poor diet causes normal age-related development to go awry.Conclusion: Poor nutrition has major consequences for the expression of genes underlying the physiology and age-related development of nurse worker bees. More work is certainly needed to fully understand the consequences of starvation and the complex biology of nutrition and development in this system, but the genes identified in the present study provide a starting point for understanding the consequences of poor diet and for mitigating the economic costs of colony starvation.