Efficient phase separation and product recovery in organic‐aqueous bioprocessing using supercritical carbon dioxide
Efficient phase separation and product recovery in organic‐aqueous bioprocessing using supercritical carbon dioxide
复制标题
使用超临界二氧化碳进行有机水生物处理的有效相分离和产物回收
DOI：
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发表时间：
2010
影响因子：
3.8
通讯作者：
A. Schmid
中科院分区：
文献类型：
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作者：
Christoph Brandenbusch;Bruno Bühler;P. Hoffmann;G. Sadowski;A. Schmid
重组微生物催化的双相烃功能化已被证明是在工业规模上取代常见化学合成路线的最有前途的方法之一。然而，稳定乳液的形成使下游加工变得复杂，尤其是相分离。这一事实已被证明是工业实施的主要障碍。为了克服这一限制，我们使用超临界二氧化碳 (scCO2) 进行相分离和产物纯化。这种稳定的乳液源自苯乙烯立体定向环氧化为(S)-氧化苯乙烯，这是一种由重组大肠杆菌催化的反应，可以有效且不可逆地去稳定，从而在几分钟内实现完全相分离。通过进一步使用 scCO2 作为萃取剂，可以在一个萃取步骤中获得纯度为 81% (w/w) 的产品 (S)-氧化苯乙烯。通过将相分离和使用 scCO2 的产物纯化相结合，必要的后处理步骤的数量可以减少到一个。这种高效且易于使用的技术通常适用于双相生物催化烃功能化的后处理，并且即使在大规模的情况下也能够实现具有成本效益的下游加工。生物技术。生物工程。 2010；107：642–651。 © 2010 Wiley 期刊公司。
Biphasic hydrocarbon functionalizations catalyzed by recombinant microorganisms have been shown to be one of the most promising approaches for replacing common chemical synthesis routes on an industrial scale. However, the formation of stable emulsions complicates downstream processing, especially phase separation. This fact has turned out to be a major hurdle for industrial implementation. To overcome this limitation, we used supercritical carbon dioxide (scCO2) for both phase separation and product purification. The stable emulsion, originating from a stereospecific epoxidation of styrene to (S)‐styrene oxide, a reaction catalyzed by recombinant Escherichia coli, could be destabilized efficiently and irreversibly, enabling complete phase separation within minutes. By further use of scCO2 as extraction agent, the product (S)‐styrene oxide could be obtained with a purity of 81% (w/w) in one single extraction step. By combining phase separation and product purification using scCO2, the number of necessary workup steps can be reduced to one. This efficient and easy to use technique is generally applicable for the workup of biphasic biocatalytic hydrocarbon functionalizations and enables a cost effective downstream processing even on a large scale. Biotechnol. Bioeng. 2010;107:642–651. © 2010 Wiley Periodicals, Inc.