New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase
New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase
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DOI：
10.1016/j.bbrc.2011.04.095
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发表时间：
2011-05-20
影响因子：
3.1
通讯作者：
Ito, Susumu
中科院分区：
文献类型：
--
作者：
Senoura, Takeshi;Ito, Shigeaki;Ito, Susumu
脆弱类杆菌NCTC 9343基因组中的BF0771-BF0774基因构成操纵子。BF0772的功能分析表明，该基因编码一种新的催化反应的酶，即4-O-β-D-甘露糖基-D-葡萄糖+PI->；甘露糖-1-磷酸+葡萄糖。在这里，我们提出了一个新的途径，包括1，4-β-甘露聚糖酶(BF0771)、甘露糖和/或糖转运蛋白(BF0773)、甘露糖2-异构体酶(BF0774)和甘露糖葡萄糖磷酸化酶(BF0772)，最终进行糖酵解。此途径分布于杆菌、副杆菌、黄杆菌和(C)2011 Elsevier Inc.等微生物中。保留所有权利。
The consecutive genes BF0771-BF0774 in the genome of Bacteroides fragilis NCTC 9343 were found to constitute an operon. The functional analysis of BF0772 showed that the gene encoded a novel enzyme, mannosylglucose phosphorylase that catalyzes the reaction, 4-O-beta-D-mannopyranosyl-D-glucose + Pi -> mannose-1-phosphate + glucose. Here we propose a new mannan catabolic pathway in the anaerobe, which involves 1,4-beta-mannanase (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772), finally progressing to glycolysis. This pathway is distributed in microbes such as Bacteroides, Parabacteroides, Flavobacterium, and (C) 2011 Elsevier Inc. All rights reserved.