Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells
Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells
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DOI：
10.1016/j.reactfunctpolym.2017.12.019
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发表时间：
2018-03-01
影响因子：
5.1
通讯作者：
Kellomaki, Minna
中科院分区：
文献类型：
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作者：
Karvinen, Jennika;Joki, Tiina;Kellomaki, Minna
再生医学，特别是细胞疗法与支持性生物材料支架相结合，被认为是人类各种缺陷的潜在治疗方法。在这里，我们已经产生并研究了可注射的腙交联透明质酸-聚乙烯醇（HA-PVA）和藻酸盐-聚乙烯醇（AL-PVA）水凝胶的详细性质，以用作3D神经细胞培养的支持性生物材料。据我们所知，这是第一次报道了腙交联的AL-PVA水凝胶的聚合和性能。报道了聚合物组分的取代度和分子量以及水凝胶的聚合物浓度对水凝胶的溶胀、降解和机械性能的影响。此外，我们研究了上述参数对人多能干细胞衍生的神经元细胞生长的影响。最神经细胞支持的HA-PVA水凝胶由具有脑模拟机械性质的高分子量HA组分和降低的聚合物浓度组成。AL-PVA水凝胶的硬度与脑组织非常相似，也显示出类似的支持性。在最软的水凝胶内，神经元的伸展和3D网络的形成得到了增强。
Regenerative medicine, especially cell therapy combined with a supportive biomaterial scaffold, is considered to be a potential treatment for various deficits in humans. Here, we have produced and investigated the detailed properties of injectable hydrazone crosslinked hyaluronan-polyvinyl alcohol (HA-PVA) and alginate-polyvinyl alcohol (AL-PVA) hydrogels to be used as a supportive biomaterial for 3D neural cell cultures. To the best of our knowledge, this is the first time the polymerization and properties of hydrazone crosslinked AL-PVA hydrogel have been reported. The effect of the degree of substitution and molecular weight of the polymer components as well as the polymer concentration of the hydrogel on the swelling, degradation and mechanical properties of the hydrogels is reported. Furthermore, we studied the effect of the above parameters on the growth of human pluripotent stem cell-derived neuronal cells. The most neural cell supportive HA-PVA hydrogel was composed of high molecular weight HA component with brain-mimicking mechanical properties and decreased polymer concentration. AL-PVA hydrogel, with stiffness quite similar to brain tissue, was also shown to be similarly supportive. Neuronal spreading and 3D network formation was enhanced inside the softest hydrogels.