Transcriptome analysis of nematode-responsive genes in two susceptible Indica rice cultivars
Transcriptome analysis of nematode-responsive genes in two susceptible Indica rice cultivars
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两个感病籼稻品种线虫响应基因的转录组分析
DOI：
10.1163/15685411-bja10098
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发表时间：
2021-04
期刊：
影响因子：
1.2
通讯作者：
Kan Zhuo
中科院分区：
文献类型：
--
作者：
Jiansong Chen;Borong Lin;Zhiwen Li;Qiuling Huang;Jinling Liao;Kan Zhuo
禾生根结线虫是危害水稻的主要植物病原菌。改变植物的敏感基因，关键是促进兼容性被认为是最有效的策略之一，以控制植物病原体。然而，目前没有关于M的资料。籼稻品种中禾生菌的感病基因本研究利用转录组分析技术，对感病程度不同的两个籼稻品种‘贵农占’和‘巫山四苗（R534）’的线虫反应基因进行了研究。在接种后3天（dpi），对禾生菌属进行了检测。在籼稻基因组中，共有33157个转录本对应于预测的基因。其中，在‘桂农占’和‘乌山丝苗’中分别鉴定出2062和1386个差异表达基因。聚类分析结果表明，在‘桂农占’和‘五山四苗’中，共有2184个DEG基因受到共同调控，503个DEG基因受到差异调控。这些DEG参与防御反应、Ca ~（2+）信号通路、细胞壁、激素通路、泛素蛋白酶体系统和转录因子。此外，还对可能参与水稻防御反应和感病的候选基因进行了研究。通过实时荧光定量RT-PCR技术对其中的部分菌株进行了进一步的验证。这些转录数据揭示了线虫感染后感病水稻基因表达模式的变化，并提供了一组新的候选籼稻品种的防御相关和感病基因。禾生菌
Meloidogyne graminicola is a major plant pathogen damaging rice. Altering a plant susceptibility gene that critically facilitates compatibility is considered one of the most effective strategies to control plant pathogens. However, no resource is currently available regarding M. graminicola susceptibility genes in Indica rice cultivars. In this study, transcriptome analysis was used to elucidate nematode-responsive genes to the two susceptible Indica rice cultivars ‘Guinongzhan’ and ‘Wushansimiao (R534)’ with distinct levels of susceptibility to M. graminicola at 3 days post-inoculation (dpi). A total of 33157 transcripts corresponded to the predicted genes in the Indica rice genome. Among these, 2062 and 1386 differentially expressed genes (DEGs) were identified in ‘Guinongzhan’ and ‘Wushansimiao (R534)’, respectively, as a result of nematode infection. Cluster analysis showed that 2184 DEGs were commonly regulated and 503 DEGs were differentially regulated in ‘Guinongzhan’ and ‘Wushansimiao (R534)’. These DEGs were involved in defence responses, the Ca2+ signal pathway, cell wall, hormone pathways, the ubiquitin proteasome system and transcription factors. In addition, candidate genes that may be involved in rice defence responses and susceptibility to M. graminicola were identified, and some of them were further validated by quantitative real-time RT-PCR. The transcription data reveal insights into the susceptible rice gene expression pattern changes upon nematode infection, and provide a novel set of candidate defence-related and susceptibility genes in Indica rice cultivars for M. graminicola.