LOCAL AND GLOBAL DYNAMICS DURING THE FOLDING OF ESCHERICHIA-COLI DIHYDROFOLATE-REDUCTASE BY TIME-RESOLVED FLUORESCENCE SPECTROSCOPY
LOCAL AND GLOBAL DYNAMICS DURING THE FOLDING OF ESCHERICHIA-COLI DIHYDROFOLATE-REDUCTASE BY TIME-RESOLVED FLUORESCENCE SPECTROSCOPY
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DOI：
10.1021/bi00006a007
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发表时间：
1995-02-14
期刊：
影响因子：
2.9
通讯作者：
MATTHEWS, CR
中科院分区：
文献类型：
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作者：
JONES, BE;BEECHEM, JM;MATTHEWS, CR
利用时间分辨荧光技术研究了二氢叶酸还原酶（DHFR）的动力学复性反应。测量的发射和各向异性衰减寿命的5个固有的色氨酸残基和荧光探针1苯胺基萘-8-磺酸盐（ANS）在折叠反应过程中被用来表征的紧凑性和发展的三级结构的瞬态中间体在折叠过程中形成的DHFR。实验监测绑定ANS显示，一个快速形成的中间体（
Time-resolved fluorescence techniques were utilized to monitor the kinetic refolding reaction of Escherichia call dihydrofolate reductase (DHFR). Measurements of emission and anisotropy decay lifetimes of both the five intrinsic tryptophan residues and the fluorescent probe 1 anilinonaphthalene-8-sulfonate (ANS) during the folding reaction were used to characterize the compactness and development of tertiary structure in transient intermediates formed during the folding of DHFR. Experiments monitoring bound ANS show that a rapidly-formed intermediate (