Continuous-Flow Magnetic Cell Sorting Using Soluble Immunomagnetic Label
Continuous-Flow Magnetic Cell Sorting Using Soluble Immunomagnetic Label
复制标题
使用可溶性免疫磁性标签进行连续流磁性细胞分选
DOI：
10.1007/978-1-4757-6482-6_17
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
J. Chalmers
中科院分区：
文献类型：
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作者：
M. Zborowski;L. Moore;Liping Sun;J. Chalmers
可溶性免疫磁性标记在细胞分选中的应用提供了与细胞表面受体密度成比例的结合的潜在优势，从而提供了高灵敏度。这些优点可以在基于连续、稳定的细胞分离的磁性细胞分选系统中完全实现，而不是像今天所实践的那样通过在磁性表面上沉积细胞来实现。设计、建造并测试了基于偶极磁场和四极磁场的两种连续流磁细胞分选系统。细胞模型体系由人外周血淋巴细胞、小鼠抗人细胞毒T细胞单抗荧光素和大鼠抗鼠免疫磁性胶体组成。用流式细胞仪检测磁疗前后细胞毒性T细胞的浓度变化。初步结果表明，磁性免疫胶体标记后的连续流动细胞分离对细胞标记的群体分布很敏感。
Soluble immunomagnetic labels in application to cell sorting offer potential advantages of binding in proportion to cell surface receptor density, and thus high sensitivity. These advantages could be fully realized in magnetic cell sorting systems based on continuous, steady-state cell separation rather than by cell deposition on magnetic surfaces as it is practiced today. Two continuous-flow magnetic cell sorting systems based on the dipole and the quadrupole magnetic fields were designed, built and tested. The cell model system comprised human peripheral lymphocytes, mouse anti-human cytotoxic T cell monoclonal antibody-fluorescein, and rat anti-mouse immunomagnetic colloid. Changes in the fractional concentration of the cytotoxic T cells before and after magnetic fractionation were measured by flow cytometry. The preliminary results indicated sensitivity of the continuous-flow cell separation following magnetic immunocolloid labeling to population distribution of the cell label.