Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay
Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay
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DOI：
10.1007/978-1-0716-0211-9_9
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Hashem, Anwar M.
中科院分区：
文献类型：
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作者：
Algaissi, Abdullah;Hashem, Anwar M.
微中和（MN）测定是病毒学、免疫学和流行病学中的一项标准且重要的技术。它是一种高度特异性和灵敏的检测方法，用于评估人和动物血清中病毒特异性中和抗体 (nAb)。它为个人或动物是否具有可以中和或抑制特定病毒株感染性的抗体提供了最精确的答案。然而，使用基于活病毒的 MN 检测可能需要在高度封闭的设施下进行，尤其是在处理中东呼吸综合征冠状病毒 (MERS-CoV) 等高风险病原体时。在本章中，我们描述了 MERS-CoV 的分离、扩增和滴定，以及用于测量不同哺乳动物物种血清中 nAb 水平的详细 MN 测定。
The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species.