Expression of Snail and E-cadherin in Drug-resistant MCF-7/ADM Breast Cancer Cell Strains
Expression of Snail and E-cadherin in Drug-resistant MCF-7/ADM Breast Cancer Cell Strains
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DOI：
10.29271/jcpsp.2019.03.240
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发表时间：
2019-03-01
影响因子：
1
通讯作者：
Tang, Jinhai
中科院分区：
文献类型：
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作者：
Cai, Fenglin;Xiao, Hualin;Tang, Jinhai
目的：探讨锌指转录因子Snail和E-cadherin在阿霉素耐药人乳腺癌MCF-7/ADM细胞和非耐药人乳腺癌MCF-7细胞中的表达。研究设计：实验研究。研究地点和时间：南京医科大学附属肿瘤医院、江苏省肿瘤医院和江苏省肿瘤研究所，2017年4月至2018年3月。方法：采用实时定量PCR技术检测正常乳腺细胞、阿霉素耐药人乳腺癌MCF-7/ADM细胞和非耐药MCF-7细胞中Snail mRNA和E-cadherin mRNA的表达水平。Western blot检测Snail和E-cadherin蛋白在正常乳腺细胞、耐药人乳腺癌MCF-7/ADM细胞和非耐药人乳腺癌MCF-7细胞中的表达水平。结果：Snail mRNA和蛋白在耐药人乳腺癌MCF-7/ADM细胞中的表达均显著高于正常乳腺细胞（P < 0.05），在耐药人乳腺癌MCF-7/ADM细胞中的表达均显著高于非耐药人乳腺癌MCF-7/ADM细胞（P < 0.05）。
Objective: To investigate the expression of zinc finger transcription factors-Snail and E-cadherin in adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.Study Design: An experimental study.Place and Duration of Study: The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital, and Jiangsu Institute of Cancer Research, China, from April 2017 to March 2018.Methodology: Real-time quantitative PCR technology was used to detect the expression levels of Snail mRNA and E-cadherin mRNA in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells. Western blot was used to detect the expression levels of proteins of Snail and E-cadherin in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.Results: The expression of Snail mRNA and protein in adriamycin-resistant human breast cancer MCF-7/ADM cells was significantly higher than that in normal breast cells (p