Analysis of antigens targeted by circulating IgG and IgA autoantibodies in 50 patients with cicatricial pemphigoid
Analysis of antigens targeted by circulating IgG and IgA autoantibodies in 50 patients with cicatricial pemphigoid
复制标题
DOI：
10.1016/s0923-1811(97)00067-4
复制
发表时间：
1998-05-01
影响因子：
4.6
通讯作者：
Hashimoto, T
中科院分区：
文献类型：
--
作者：
Murakami, H;Nishioka, S;Hashimoto, T
在这项研究中，我们调查了50例典型的瘢痕性类天疱疮（CP）患者的血清。通过对1 M氯化钠分裂的人皮肤切片进行间接免疫荧光，17份血清的IgG和22份血清的伊加与分裂的表皮侧反应，而两份血清的IgG与真皮侧反应。后两种血清后来被证实是抗epiligrin CP。用免疫印迹法检测14份血清IgG与230 kD大疱性类天疱疮抗原（BP 230）的反应。15份血清IgG和11份血清伊加与180 kD BP抗原（BP 180）反应。有趣的是，含有BP 180 NC 16 a结构域的细菌融合蛋白被18份血清的IgG识别，但不被任何血清的IEA识别。含有BP 180 C-末端区域的融合蛋白被20份血清的IgG识别，但仅被2份血清的IEA检测到。我们的研究结果表明，虽然CP血清显示非常低滴度的自身抗体，相当数量的血清含有IgG抗体BP 180（NC 16 a或C-末端结构域），证实了以前的研究。此外，我们发现，更多数量的伊加抗体与BP 180反应，似乎与IgG抗体不同类型的表位。由于IgG抗体的特异性与BP的特异性没有很大差异，伊加抗体可能对CP特征性临床特征的发展起特定作用。未来的研究应阐明伊加抗体在CP中的致病作用。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
In this study we investigated sera from 50 typical cicatricial pemphigoid (CP) patients. By indirect immunofluorescence on 1 M NaCl-split human skin sections, IgG of 17 sera and IgA of 22 sera reacted with the epidermal side of the split, while IgG of two sera reacted with the dermal side. These latter two sera were later confirmed to be anti-epiligrin CP. By immunoblotting of epidermal extracts, IgG of 14 sera reacted with the 230 kD bullous pemphigoid (BP) antigen (BP230). IgG of 15 sera and IgA of 11 sera reacted with the 180 kD BP antigen (BP180). Interestingly, a bacterial fusion protein containing the BP180 NC16a domain was recognized by IgG of 18 sera but not by IEA of any sera. Fusion proteins containing the C-terminal region of BP180 were recognized by IgG of 20 sera, but it was detected by IEA of only two sera. Our results suggest that, although CP sera show very low titers of autoantibodies, a considerable number of sera contain IgG antibodies to BP180 (either NC16a or C-terminal domain), confirming previous studies. In addition, we showed that greater numbers of IgA antibodies react with BP180, seemingly with different types of epitopes from those for IgG antibodies. Because the specificity of IgG antibodies is not very different from those in BP, IgA antibodies may play a specific role for the development of characteristic clinical features in CP. Future studies should elucidate the pathogenic role of the IgA antibodies in CP. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.