The role of actin cables in directing the morphogenesis of the pharyngeal pouches
The role of actin cables in directing the morphogenesis of the pharyngeal pouches
复制标题
肌动蛋白缆在指导咽袋形态发生中的作用
DOI：
10.1242/dev.00950
复制
发表时间：
2004
影响因子：
3.9
通讯作者：
A. Graham
中科院分区：
文献类型：
--
作者：
R. Quinlan;Paul Martin;A. Graham
咽弓被内胚层外袋（咽袋）分隔开。这些都是相当重要的结构；它们需要分离每个弓的间充质群体并诱导弓成分的形成，并且它们产生特定的衍生物，包括甲状旁腺和胸腺。咽袋首先作为内胚层与外胚层接触的局部部位出现，然后沿近远轴扩张，形成成熟袋的狭窄、紧密的形态。我们目前对指导咽袋形成的形态发生机制一无所知。在这里，在小鸡中，我们发现咽袋内胚层内的细胞具有丰富的位于顶端的肌动蛋白纤维，这些肌动蛋白纤维通过插入 N-钙粘蛋白粘附连接而在内胚层内联网，形成细胞上肌动蛋白电缆网。细胞松弛素 D 破坏这些肌动蛋白结构，导致形成异常的囊袋，无法产生正常的狭缝状形态。这表明咽袋形态发生的过程涉及这些肌动蛋白电缆的约束影响，这些肌动蛋白电缆在咽袋内沿着近远轴引导扩张。重要的是，这些结果为我们提供了关于咽袋如何形成其正常形态的重要见解。他们还首次提供了肌动蛋白电缆在复杂的脊椎动物形态发生事件中发挥约束作用的证据。
The pharyngeal arches are separated by endodermal outpocketings, the pharyngeal pouches. These are structures of considerable importance; they are required to segregate the mesenchymal populations of each arch and to induce the formation of arch components, and they generate specific derivatives, including the parathyroid and the thymus. The pharyngeal pouches are first evident as localised sites at which the endoderm contacts the ectoderm, and they then expand along the proximodistal axis to generate the narrow, tight morphology of the mature pouch. We currently have no knowledge of the morphogenetic mechanisms that direct formation of the pharyngeal pouches. Here, in chick, we show that cells within the pharyngeal pouch endoderm have an abundance of apically located actin fibres that are networked within the endodermal sheet, via their insertion into N-cadherin adherens junctions, to form a web of supra-cellular actin cables. Cytochalasin D disruption of these actin structures results in the formation of aberrant pouches that fail to generate their normal slit-like morphology. This suggests that the process of pharyngeal pouch morphogenesis involves the constraining influence of these actin cables that direct expansion, within the pouch, along the proximodistal axis. These results, importantly, provide us with vital insights into how the pharyngeal pouches form their normal morphology. They also give evidence, for the first time, of actin cables functioning as constraints during complex vertebrate morphogenetic episodes.
果蝇 RhoA 调节发育中表皮的细胞骨架和细胞间粘附。
DOI：
10.1242/dev.129.13.3173
发表时间：
2002
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Bloor,JamesW;Kiehart,DanielP
通讯作者：
Kiehart,DanielP