Bacteriophage WO-B and Wolbachia in natural mosquito hosts:: infection incidence, transmission mode and relative density
Bacteriophage WO-B and Wolbachia in natural mosquito hosts:: infection incidence, transmission mode and relative density
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DOI：
10.1111/j.1365-294x.2006.02947.x
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发表时间：
2006-08-01
影响因子：
4.9
通讯作者：
Kittayapong, P. .
中科院分区：
文献类型：
--
作者：
Chauvatcharin, N.;Ahantarig, A.;Kittayapong, P. .
沃尔巴克氏体噬菌体已被提出作为一种潜在的转化工具，用于遗传修饰蚊子载体。在这项研究中，我们报告了几种蚊子宿主的自然种群中存在WO-B类沃尔巴克氏体相关的寄生虫。88%（22/25）的受Wolbachia感染的蚊子被发现含有WO-B。WO-B噬菌体orf 7序列分析表明，大多数单感染（23/24）或双感染（1/1）的蚊子体内存在单一的WO-B噬菌体。然而，发现感染复杂伊蚊的单个沃尔巴克氏体菌株具有至少两种不同的WO-B。系统发育分析表明，WO-B的水平传播发生在蚊子体内的沃尔巴克氏体之间的进化尺度上。在生态尺度上，共传播的低趋势发生在特定的WO-B之间的Wolbachia的每种蚊子。通过实时定量聚合酶链反应（RTQ-PCR）评估WO-B噬菌体的密度显示，对于单个Wolbachia菌株感染的蚊子，每只蚊子的平均相对密度为7.76 x 10（5）+/- 1.61 x 10（5）orf 7拷贝，但在双重Wolbachia感染的白纹伊蚊中的密度要高出3倍。然而，WO-B噬菌体的平均组合密度与其Wolbachia宿主的平均组合密度不相关，这在不同的蚊子物种中是不同的。我们还证实了在实验室的Ae菌落WO-B样病毒颗粒的存在。白纹伊蚊（KLPP）的形态，通过透射电子显微镜（TEM）。Ae经纯化、过滤后可检出病毒样颗粒。白纹伊蚊卵巢提取物，表明至少一种WO-B样噬菌体在该蚊子载体的沃尔巴克氏体内是活性的（温和的）。然而，利用这些噬菌体作为转化载体的想法仍然需要更多的研究，可能是不可行的。
Bacteriophages of Wolbachia bacteria have been proposed as a potential transformation tool for genetically modifying mosquito vectors. In this study, we report the presence of the WO-B class of Wolbachia-associated phages among natural populations of several mosquito hosts. Eighty-eight percent (22/25) of Wolbachia-infected mosquito species surveyed were found to contain WO-B phages. WO-B phage orf7 sequence analysis suggested that a single strain of WO-B phage was found in most singly (23/24) or doubly (1/1) Wolbachia-infected mosquitoes. However, the single Wolbachia strain infecting Aedes perplexus was found to harbour at least two different WO-B phages. Phylogenetic analysis suggested that horizontal transmission of WO-B phages has occurred on an evolutionary scale between the Wolbachia residing in mosquitoes. On an ecological scale, a low trend of co-transmission occurred among specific WO-B phages within Wolbachia of each mosquito species. Assessment of the density of WO-B phage by real-time quantitative polymerase chain reaction (RTQ-PCR) revealed an average relative density of 7.76 x 10(5) +/- 1.61 x 10(5) orf7 copies per individual mosquito for a single Wolbachia strain infecting mosquitoes, but a threefold higher density in the doubly Wolbachia-infected Aedes albopictus. However, the average combined density of WO-B phage(s) did not correlate with that of their Wolbachia hosts, which varied in different mosquito species. We also confirmed the presence of WO-B-like virus particles in the laboratory colony of Ae. albopictus (KLPP) morphologically, by transmission electron microscopy (TEM). The viral-like particles were detected after purification and filtration of Ae. albopictus ovary extract, suggesting that at least one WO-B-like phage is active (temperate) within the Wolbachia of this mosquito vector. Nevertheless, the idea of utilizing these bacteriophages as transformation vectors still needs more investigation and is likely to be unfeasible.