Polychromatic fingerprinting of excitation emission matrices
Polychromatic fingerprinting of excitation emission matrices
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DOI：
10.1002/chem.200800590
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Filippini, Daniel
中科院分区：
文献类型：
--
作者：
Gatto, Emanuela;Malik, Muhammad A.;Filippini, Daniel
普遍存在的多色激发支持荧光指示剂的激发发射矩阵（EEM）的指纹识别的能力进行了报道。制备荧光分子溶液，浓度为2µM用于吸收测量，10µM用于发射测量。使用通过Milli-Q纯化系统的光谱级THF和蒸馏水作为溶剂。EEM测量在荧光分光光度计上进行，在390-700 nm检测范围内操作，在相同范围内以20 nm间隔激发。在测量期间，屏幕显示50种颜色的序列，并且对于每种颜色，记录参考和样品的透射和发射光谱。红色区域的照明颜色不能激发荧光，这可以在红光的光谱中看到。
The ability of a ubiquitous polychromatic excitation to support the fingerprinting of the Excitation Emission Matrices (EEM) of fluorescent indicators was reported. Solutions of fluorescent molecules, with concentration of 2µM for the absorption and 10µM for the emission measurements were prepared. Spectroscopic grade THF and distilled water passed through Milli-Q purification system were used as solvents. EEM measurements were carried out on a spectrofluorimeter, operating in the 390-700 nm detection range, with excitations at 20 nm intervals within the same range. During the measurement, the screen displayed a sequence of 50 colors and for every color, the transmission and emission spectrum of both the reference and the sample were recorded. Illuminating colors in the red region are not able to excite fluorescence and this can be seen in the spectra for red light.