Differential expression of Toll-like receptors in follicular lymphoma, diffuse large B-cell lymphoma and peripheral T-cell lymphoma
Differential expression of Toll-like receptors in follicular lymphoma, diffuse large B-cell lymphoma and peripheral T-cell lymphoma
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DOI：
10.1016/j.yexmp.2010.08.003
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发表时间：
2010-12-01
影响因子：
3.6
通讯作者：
Kitagawa, Masanobu
中科院分区：
文献类型：
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作者：
Smith, Thomas J.;Yamamoto, Kouhei;Kitagawa, Masanobu
尽管众所周知，哺乳动物中的 Toll 样受体 (TLR) 在先天免疫中发挥重要作用，但 TLR 的新作用表明，TLR 表达异常的细胞可能比正常细胞具有生存优势。淋巴细胞是表达许多 TLR 的少数细胞类型之一，表明异常的 TLR 水平/信号传导可能会影响恶性淋巴瘤的进展。因此，使用定量实时 PCR 分析了来自滤泡性淋巴瘤 (FL)、弥漫性大 B 细胞淋巴瘤 (DLBCL) 和外周 T 细胞淋巴瘤 (PTCL) 患者的 51 个淋巴结的冷冻样本中 TLR1 至 9 的表达，并与反应性淋巴结病 (RL) 样本中的表达进行比较。与 RL 相比，TLR2 在 DLBCL 和 PTCL 中均过表达，但在 FL 中则不然。PTCL 中 TLR1 和 TLR4 表达上调，而 TLR8 在 DLBCL 中高表达。尽管 TLR5 在 FL 中表达较低，但不同淋巴瘤亚型之间 TLR3、TLR6、TLR7 和 TLR9 的表达没有显着差异。双重免疫染色显示，DLBCL 中表达 TLR2 和/或 TLR8 的淋巴瘤细胞数量增加。在 PTCL 中，TLR2 和 TLR4 表达局限于肿瘤性 T 细胞。 TLR 表达在淋巴瘤亚型之间差异很大。然而，尽管如此，仍然存在一些显着差异，这些差异可能有助于开发新的治疗策略。 (C) 2010 Elsevier Inc. 保留所有权利。
Although Toll-like receptors (TLRs) in mammals are well-known to play important roles in innate immunity, newer roles for the TLRs have suggested that cells with aberrant TLR expression may have a survival advantage over normal cells. Lymphocytes are one of a small number of cell types that express many of the TLRs, suggesting that abnormal TLR levels/signaling may potentially influence the progression of malignant lymphomas. Thus, frozen samples of 51 lymph nodes from patients with follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL) and peripheral T-cell lymphoma (PTCL) were analyzed for the expression of TLR1 to 9 using quantitative real-time PCR, and compared to those in reactive lymphadenopathy (RL) samples. TLR2 was over-expressed in both DLBCL and PTCL but not in FL when compared to RL TLR1 and TLR4 expression was up-regulated in PTCL, while TLR8 was highly expressed in DLBCL Although TLR5 showed lower expression in FL, expression of TLR3, TLR6, TLR7 and TLR9 did not vary significantly between different lymphoma subtypes. Double immunostaining revealed an increase in the number of TLR2 and/or TLR8 expressing lymphoma cells in DLBCL In PTCL TLR2 and TLR4 expression was localized to neoplastic T cells. TLR expression is highly variable among lymphoma subtypes. However, despite this some significant differences exist that may prove useful in the development of novel therapeutic strategies. (C) 2010 Elsevier Inc. All rights reserved.