Cyclic adenosine 3',5'-monophosphate-induced ovulation in the perfused rat ovary and its mediation by prostaglandins.
Cyclic adenosine 3',5'-monophosphate-induced ovulation in the perfused rat ovary and its mediation by prostaglandins.
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环腺苷 3,5-单磷酸诱导灌注大鼠卵巢排卵及其通过前列腺素的介导。
DOI：
10.1095/biolreprod37.5.1047
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发表时间：
1987
影响因子：
3.6
通讯作者：
P. Janson
中科院分区：
文献类型：
--
作者：
M. Brännström;R. Koos;W. L. Le Maire;P. Janson
本文用离体灌流大鼠卵巢模型研究了环磷酸腺苷（cAMP）在排卵过程中的作用及其机制。孕马血清促性腺激素（PMSG，20 IU）致敏大鼠卵巢灌注21小时开始在诱导发情前期的早晨。用促黄体生成素（LH; 0.1微克/毫升）进行体外刺激导致每个处理卵巢排卵2.4 +/- 0.7次。通过添加毛喉素（30 μ M）或二丁酰cAMP（dbcAMP，1 mM）和异丁基甲基黄嘌呤（IBMX，0.2 mM）也可以诱导排卵，每个处理的卵巢分别有11.8 +/- 1.9和18.6 +/- 4.4个排卵。吲哚美辛（5 μ g/ml）显著降低了毛喉素和dbcAMP + IBMX组的排卵数。向毛喉素+吲哚美辛组中加入前列腺素E2（PGE 2; 1 μ g/ml，在灌注期间三次）逆转了对排卵的抑制（每个处理卵巢21.6 +/- 5.4次排卵）。卵巢PGE组织水平显着较高的LH，毛喉素，或dbcAMP + IBMX刺激后10小时相比，未经刺激的对照组。LH和毛喉素组中的排卵卵母细胞恢复减数分裂，但dbcAMP + IBMX组中的卵母细胞仍然不成熟。这项研究表明，卵巢cAMP的增加，即使不是由LH诱导的，也足以引起排卵前大鼠卵泡的排卵，支持cAMP参与PMSG处理的大鼠的正常排卵过程。此外，前列腺素参与cAMP诱导的排卵被证明。
The role and mechanism of action of cyclic adenosine 3',5'-monophosphate (cAMP) in the ovulatory process was investigated by using the in vitro-perfused rat ovary model. Ovaries of pregnant mare's serum gonadotropin (PMSG, 20 IU)-primed rats were perfused for 21 h beginning in the morning of induced proestrus. In vitro stimulation with luteinizing hormone (LH; 0.1 micrograms/ml) resulted in 2.4 +/- 0.7 ovulations per treated ovary. Ovulations could also be induced by the addition of forskolin (30 microM) or dibutyryl cAMP (dbcAMP, 1 mM) with isobutylmethylxanthine (IBMX, 0.2 mM), with 11.8 +/- 1.9 and 18.6 +/- 4.4 ovulations per treated ovary, respectively. Indomethacin (5 micrograms/ml) significantly decreased the number of ovulations in the forskolin and dbcAMP + IBMX groups. The addition of prostaglandin E2 (PGE2; 1 micrograms/ml three times during the perfusion) to the forskolin + indomethacin group reversed the inhibition of ovulation (21.6 +/- 5.4 ovulations per treated ovary). Ovarian PGE tissue levels were significantly higher 10 h after stimulation with either LH, forskolin, or dbcAMP + IBMX compared to the unstimulated control group. Ovulated oocytes in the LH and forskolin groups resumed meiosis but oocytes in the dbcAMP + IBMX groups remained immature. This study shows that an increase in ovarian cAMP, even if not induced by LH, is sufficient to cause ovulation of preovulatory rat follicles, supporting the involvement of cAMP in the normal ovulatory process of the PMSG-treated rat. Furthermore, prostaglandin involvement in cAMP-induced ovulations is demonstrated.